FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4377946
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT424412396.94324690162921No Hit
CTAGCTGA251230.57385358339276No Hit
CTAGCAGT95040.2170881047870394No Hit
CTAGCTGG88390.2018983331452695No Hit
CAAGCTGT83590.19093428744895438No Hit
CTAGCGGT82340.18807906721553896No Hit
CTAGATGT75890.17334613081111552No Hit
CTAGCTAT75620.1727294032406978RNA PCR Primer, Index 38 (100% over 8bp)
CTCGCTGT71290.1628389203521469No Hit
CTAGCTCT68910.15740258102772395No Hit
CTACCTGT63350.14470256142949228No Hit
ATAGCTGT56090.12811944231381564No Hit
CTAGGTGT47440.10836131829858112No Hit
CTAGCTTT45500.10393001649632043No Hit
CTTGCTGT44380.10137173916718023No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)