FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31234203
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG399830.12801030972360652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG37050.08.3847545
GTGTATA59400.08.361991
TAGACTG43200.08.1952535
TACACTG58700.08.1529285
TAGACCG11400.07.97873545
TCTACAC43450.07.8567463
AGGACCG33200.07.7975695
GTCCTAG45900.07.7426351
TAGACAG51350.07.684835
TCTAGAC37900.07.6414153
CTACACT54850.07.6019834
CTAGGAC48650.07.59213163
TACACCG15500.07.58356055
CTTATAC26950.07.57943343
TACACAG71300.07.5757115
TCTATAC26400.07.4723613
GTATTAG33500.07.3508171
CTAGTAC21000.07.3285163
TGGACTG52800.07.2882685
TAGGACC32550.07.2647894
Produced by FastQC (version 0.11.5)