FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596475
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT345132495.96407593546459No Hit
CTTTCCTA653041.815777949241966No Hit
CTTTCCAT99760.2773827150195678No Hit
CTTTACTT95460.26542656351010363No Hit
CTTTCATT87360.24290451066669447No Hit
CATTCCTT66950.18615449850200544TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT63680.17706226235411063No Hit
ATTTCCTT61860.17200175171522117No Hit
CTTACCTT50110.13933087259052265No Hit
CGTTCCTT42900.11928346505953746No Hit
CTTTCCGT37020.10293412299543303No Hit
CTGTCCTT36460.10137704279885165No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)