FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12440236
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA1214180597.60108248750264No Hit
GTGGCGCA676510.54380801135927No Hit
GTGGAGCC312610.2512894449912365No Hit
GTGGAGCT238160.19144331345482513No Hit
GAGGAGCA187880.15102607378188002No Hit
GGGGAGCA178220.14326094778266263No Hit
GTGTAGCA163270.13124349087911194No Hit
GTGGACCA143620.11544797060120081ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGGAGAA131370.1056008905297295No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)