FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979596
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT330201.1082039310027265No Hit
TGCTGCTACCGTTCTC303501.0185944671693747No Hit
AAACGACCTCTTCAAG294100.9870465660445242No Hit
ATGGGTTACAATCGAA290200.9739575432374054No Hit
TATGCTTTGGACTGTT284980.9564383896340308No Hit
GCCTCTTGATCTGTGA268200.9001220299664787No Hit
AAAGCGGACTCGGGCT265530.8911610835831436No Hit
TCCAAAGTGCTCACGG252490.8473967611716487No Hit
TAGTGCATGAACCCGC231840.7780920634878017No Hit
GCAGGTCTGGCTGTTA226350.7596667467670114No Hit
TGTGGCTCTTACGATC221770.7442955353678821No Hit
TAACTCACTGGTGTCC218450.7331530851833604No Hit
GAAGTATGAACAGGTG212170.712076402304205No Hit
TTCCCATCTCTTTGTT205350.6891873931902177No Hit
TCACCTGACTCAATGC193970.6509942958709838No Hit
TGAGAATGAGCAGTTT191190.641664171921294No Hit
GAGCCTGACCGAGTTT188000.6309580224970097No Hit
GGAACTACTACCAAGG186400.6255881669863967No Hit
CTAGAGACTAATCTGC177090.5943423202340183No Hit
ATGTCCAGAGTCGGTC172930.5803806959064249No Hit
TTCGTCCGACCTGTGG171900.5769238514214678No Hit
TCGTATACTGCTAATC169160.5677279738595433No Hit
ATTCCTAACTGCTACA168440.5653115388797676No Hit
TCACACAGATTGGGAG167110.5608478464865707No Hit
AGGTCGAGAAGGGAGG164980.5536992263380673No Hit
TCCGCGAGAGAACAGC161120.5407444499187137No Hit
ACTGATTCTCGGCTCA160600.5389992468777647No Hit
TGGACCTCTAGGGAGG157670.5291656989739548No Hit
TGGCCTGGACCCGAGT155710.5225876259734541No Hit
CCATGATGATTCGGGC154900.5198691366212064No Hit
GCAATCATGGAACGTG149490.5017123126759466No Hit
TTCGATGGAAATCCTG139700.46885550927038433No Hit
ACGAGGATGGAGCTGA134990.45304799711101773No Hit
AAGGTGCGAACAGATA134150.450228822967946No Hit
TTTCGTAACAGTACGG130580.4382473328598911No Hit
TGGTCCTTGGAGAGGG127830.4290178937010253No Hit
TCCCAGACTGTCTTGA127620.4283131001652573No Hit
TGCGGTGACCTTAGTG125480.42113091841981265No Hit
TCTCTCTACTCTGGAT122490.4110960009343549No Hit
TCCTTCACTTGTCACA109200.3664926385993269No Hit
AGCTGCTCTTGTCACA109050.36598921464520695No Hit
TGATATCCTCGATGTT108310.36350565647154853No Hit
TTCGATGGACAGGTGA107720.36152552225201No Hit
AGCCCAGTGAAAGTAC107100.3594447032416475No Hit
TCGCCGAGAAGGTAAT105770.35498101084845063No Hit
AACTCCCTGCTTCACC105560.35427621731268266No Hit
CTTTCGGTGCTTTGTT104610.3510878656032563No Hit
GAAGTAATGAGTCTGT102330.34343582150063295No Hit
GGGGGGGGGGGGGGGG99230.33303172644882056No Hit
AACCCTCACGCGGTTC98740.3313872081986954No Hit
CCCTTGCCTCCTACGG98650.33108515382622344No Hit
ATGGCCGTGATACGCT96930.3253125591523146No Hit
TCGGCTTACCTCCTAC96110.32256050820312554No Hit
AAACACGCTGCAGTTT91810.30812902151835353No Hit
TGTTTGTTGCAAGTTC90960.30527628577834043No Hit
ACCCTTGTGGGCCATC90170.30262491961997534No Hit
TGGCCTTTGCGAAAGC86420.29003932076697647No Hit
CTAACTGGAACCTTGA85400.2866160378789608No Hit
CAATGTGTGGTTGACT85220.2860119291340168No Hit
TACAACGTGAGCAGGT84620.283998233317537No Hit
AAATAGGACTCTATCT83420.27997084168457737No Hit
TCAAAGGCTCTTAGTG81640.27399687742902057No Hit
GGACATTTGGCCGCTA81010.2718824968217168No Hit
ACAGCCGACAAACATG79740.26762017401016786No Hit
CTTTCGGTGAGCCATT79170.265707162984512No Hit
TGAACTGTGTGCTGGC76310.2561085462592915No Hit
CAGCCGATGTAATAGG74120.2487585565291402No Hit
AGTGTTCGATTCGCCT73690.24731540786066297No Hit
GGCTTGCGAATACGCT68590.23019899342058453No Hit
GATAAGAACATTGGTC63240.21224353905697282No Hit
GTGCTAATGGGAGTCA63070.21167299190897024No Hit
TTGGGAGGAATCTGTG62020.20814902423013054No Hit
GTAAAGCCTGAAATCA61910.20777984666377589No Hit
TGTACTGGAAATGCTC60510.20308122309198964No Hit
AGGGACCCTAATCCTG59100.19834903792326206No Hit
CGACGTTCTTGCTACA59050.19818122993855544No Hit
GGACCACTGGCTAGAG58570.19657027328537158No Hit
GGAACATCTTTACGAC56470.1895223379276922No Hit
GGGTGAAACCGACTAT55950.18777713488674302No Hit
TAACGAGCTTACTTTC55150.18509220713143662No Hit
CGGATGAGAACAGGCA54410.18260864895777817No Hit
TCTTGAACTCGTACAT54080.18150111625871426No Hit
TACCACCTGAAACATG53800.18056139154435702No Hit
TACGTGTGAAACGCAG53620.17995728279941306No Hit
AGCTTGTGAACCGTGC52580.17646687671751474No Hit
CATGATGTGCCTAGTA52220.17525865922762685No Hit
ACTTATCTGTCAGGTT51920.1742518113193869No Hit
AACTAGCACTGCCACG48750.16361278508898522No Hit
TGGTAGGGATGTGCTA46780.15700115049154315No Hit
ACGTGTTGATCTTTGT45430.15247033490446354No Hit
CGTCGTACTTGCGCCA45080.151295679011517No Hit
AACTACGACATTGCCA44930.15079225505739705No Hit
TCGGACCGAATGTCCA44790.1503223927002184No Hit
AACGAACCTGACACCC44480.14928198319503716No Hit
GTAGATAGAGCTGAGA44380.14894636722562388No Hit
GTCCGAGTGGTCTTGA43820.1470669177969094No Hit
AGTTTGCGAGCAGGAT43230.1450867835773709No Hit
TACCACCTGTACTTTC43230.1450867835773709No Hit
CCTTCAGTGCAACGCC43160.14485185239878157No Hit
GCCATAATGACCGTTA42830.14374431969971768No Hit
CTCGGATCTAGTACGG42800.1436436349088937No Hit
GGTAGTTTGTCCAGCG42750.14347582692418703No Hit
TTTAGGGACTGGGCAA42620.14303952616394974No Hit
TGAGGAGTGGTCCCAG41530.13938131209734475No Hit
CTTTAACTGTTCGCCT41060.13780391704110223No Hit
AGAACGCGACACAGTT38910.1305881736987162No Hit
GAGGTTGGAGGAATAT38510.12924570982106298No Hit
GTCCGTTACGGATGGT38170.12810461552505778No Hit
CTTACATTGGAAGGCT37620.12625872769328458No Hit
CAAAGACCTGCTTTGA37540.12599023491775396No Hit
GAACCATACTTTGGGT36770.12340599195327152No Hit
GGGATGTGATGCATGT36520.12256695202973826No Hit
CCGTTACTGTTGGTTG36200.12149298092761569No Hit
TGCAATGACTCCGTAA36000.12082174898878908No Hit
CAGGCCATGTCGTAGT35950.12065394100408244No Hit
GAGAAGAGATGCTACA35620.11954640830501853No Hit
GAAAGTCCTTATCTGG35550.11931147712642921No Hit
CGATCGGTGCGAGGAT35260.11833819081513064No Hit
AACTACGACGCTGTTA35190.11810325963654132No Hit
AATTCTGCTTAGCGAG34620.1161902486108855No Hit
GCTGGACACATTGGTC33560.11263271933510449No Hit
TGTCTTCCTGAAATCA33290.11172655621768857No Hit
CCCACCTCTTGACCTA33010.11078683150333131No Hit
TCCTCATCTTTCTGGA32820.11014916116144605No Hit
GGCTTAAACTGCATCA32600.10941080602873679No Hit
ATAGCCAACATCTGTG32500.10907519005932348No Hit
ATGGCCGTGGGCCAAA31870.10696080945201966No Hit
TCGTAGAACCGCACTG31830.10682656306425435No Hit
ACAAGACCTAAGCTAA31820.10679300146731302No Hit
CCTGAGGACTGACTCA31700.10639026230401705No Hit
TATGGGTACCCTTTGC30740.10316834899764935No Hit
AGGTGATCTGTTAGTC30710.10306766420682535No Hit
TCCGTAAGATTACGCA30620.10276560983435339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)