FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427923
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC150040.6179767645020043No Hit
CTCCTAGTGAACGTAA127540.5253049623072891No Hit
TAGCTTTACCCTATCC113350.4668599457231551No Hit
CCAGGACTGTAACTCG84920.34976397521667696No Hit
ATCTGTACTGGGACTA82270.3388492962915216No Hit
GTTCTCGACCGTAGAG75900.3126128794035066No Hit
CTTCCTTTGGCTCAGC75360.31038875615083344No Hit
CGATCAAACCCGGCTA73270.3017805754136354No Hit
GGGGGGGGGGGGGGGG63140.26005767069219243No Hit
TGCTGCTACTTTCTTG61810.2545797374957937No Hit
ACAGCCGACTTGCGTA59420.24473593272933283No Hit
AACTTACCTCACCTAA52790.21742864168262338No Hit
CGCGTTGGAGCGGTTC50900.20964421029826727No Hit
GGCAACAGAATAGCAA49270.20293065307260566No Hit
CGACCAGACGCGCTGT48520.1998415929994485No Hit
ATCGAAAGATTTCCGC48270.19881190630839612No Hit
GTAACTGTGTGAAAGT46970.19345753551492367No Hit
AGTGGAGTGAGTTGAA46950.19337516057963947No Hit
CCCTGAACTAGCTTTG46810.19279853603265013No Hit
CAATTCGGATAACGGG45120.18583785400113595No Hit
GGGTAGAACCAGTCTA42270.17409942572313866No Hit
TTCCTAAACTAATGCC40700.16763299330332962No Hit
AAGAACCCTTAACCCA40520.16689161888577192No Hit
CGCGATCGATATCTGG39200.16145487315701526No Hit
TTTAGTCTGAGCAATA38550.15877768776027906No Hit
GTGAAGCGATCCCAGG37740.15544150288126932No Hit
TGGAACACTCCTAGTA37380.15395875404615386No Hit
TCCGGGAACGTTCTCC37270.1535056919020908No Hit
TGCAGATACTGCCACG36880.15189938066404907No Hit
CTTTAACTGTCCGTAA36040.14843963338211302No Hit
TGATAGAGAGACTGTT35400.14580363545301891No Hit
AGTCCTGCTCGAACGA34940.14390901194148248No Hit
AGGTGTGGATCATGTG34320.14135538894767258No Hit
TGCAGTATGACACCGC34150.1406552019977569No Hit
CACATCCTGAGCCTAT34100.14044926465954644No Hit
TCTCAGAGATGCATGT33940.13979026517727292No Hit
GCAACTTGAAGGTGCA33360.13740139205403137No Hit
CATAGGTACAAGGTGC33150.13653645523354735No Hit
CTATTGGGACTTGTGA32820.13517726880135822No Hit
ATTTCTGACGGGTAGT32620.13435351944851628No Hit
TACGTTTACTGCACCG32570.1341475821103058No Hit
CTTAGATCTTGTCACA32430.13357095756331647No Hit
ATGTACGTGTACTTTC31570.13002883534609622No Hit
TCAAAGCACTCTGCAA31440.12949339826674897No Hit
TGAGGGATGTACGTTG31290.12887558625211756No Hit
AGAGTACCTTAATCGC31190.1284637115756966No Hit
TGGCAATTGAGTGGTG31090.12805183689927563No Hit
TGAACTGTGAACGTAA30570.12591008858188665No Hit
GGTCGCATGGTACTAC30450.12541583897018152No Hit
CGAGTCTCTGCTACGC30290.12475683948790799No Hit
TTCTTAGACGTCCCGA30140.12413902747327654No Hit
GGGTGTGCTCGCAAAG29590.12187371675296127No Hit
TCCATTTGAAATCTGC29150.12006146817670907No Hit
TCTATGGGATGCTCAA29140.12002028070906698No Hit
GGTCTAAACTCCTACC28990.11940246869443552No Hit
TACTGTTTGCTTAAGC28960.11927890629150925No Hit
GGGATGTGACTGGAAG28570.1176725950534675No Hit
CATTCTATGCCTAGCG28240.11631340862127836No Hit
TCCGGGTGAACACCTA28200.11614865875070995No Hit
GAGTGTTACACCTGCG28140.11590153394485739No Hit
GCATGATACTTGCGTA27810.11454234751266823No Hit
CTACAACGAATCTGTG27750.11429522270681566No Hit
TTGCGGTCTTTTGGGT27740.11425403523917356No Hit
CTCAATTTGGAATCTC27650.11388334803039471No Hit
CACCAGGTGCGCCACA27630.11380097309511052No Hit
AGACTACTGGCCCTAG27420.11293603627462649No Hit
CTAGCTCACGAATAGT27200.11202991198650039No Hit
CGAGTCTCTTGGAACG26950.111000225295448No Hit
GTAATCTCTACACAAA26470.1090232268486274No Hit
CACATTACTGATGGAC26340.10848778976928015No Hit
CTTTCGTCTCGAACGA26220.10799354015757501No Hit
TCGGACCGATAGGCGA26140.10766404041643825No Hit
TGCTAGTGAAGGCGTA26110.10754047801351195No Hit
TGTAAGCACGCGCTGT25840.10642841638717539No Hit
CATAGTCGAAGATTCC25690.10581060437254393No Hit
AGCCAATTGTTATGGC25620.10552229209904926No Hit
NNNNNNNNNNNNNNNN25580.10535754222848089No Hit
ACCAAGTTGAAGCTAA25500.10502804248734411No Hit
GGCTGACTGAAACTGA25070.10325698137873401No Hit
ATGTCCAGACCGCTAG25060.10321579391109191No Hit
TGCGCAGACATCAGGG24740.10189779494654484No Hit
AGGCGATGACCTGCGA24440.10066217091728197No Hit
ACGAGGATGAACTAGA24410.1005386085143557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)