FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556720
Sequences flagged as poor quality0
Sequence length8
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA146007493.79169022046354No Hit
TTAGTCAA694314.460082738064649No Hit
TCAGGCAA43060.2766072254483787No Hit
TCAGACAA41360.26568682871678917No Hit
TCAGTAAA33290.21384706305565548No Hit
ACAGTCAA32520.20890076571252378TruSeq Adapter, Index 13 (100% over 8bp)
TAAGTCAA19340.12423557222878874No Hit
TCAGTCAT17750.11402178940336091No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)