FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004949550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17622436
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1856231.0533333757035632No Hit
AAACGACCTCTTCAAG1731910.9827869427359532No Hit
TGCTGCTACCGTTCTC1703330.9665689805881548No Hit
ATGGGTTACAATCGAA1690750.9594303534426227No Hit
TATGCTTTGGACTGTT1658500.9411298188286796No Hit
AAAGCGGACTCGGGCT1531600.8691193430919539No Hit
GCCTCTTGATCTGTGA1527630.8668665330945167No Hit
TCCAAAGTGCTCACGG1465440.8315762928575823No Hit
TAGTGCATGAACCCGC1383990.7853568031116698No Hit
GCAGGTCTGGCTGTTA1311560.7442557884732849No Hit
TAACTCACTGGTGTCC1303490.7396763988815167No Hit
TGTGGCTCTTACGATC1293120.7337918548831728No Hit
GAAGTATGAACAGGTG1281610.7272604082659174No Hit
TTCCCATCTCTTTGTT1171860.6649818447347461No Hit
TCACCTGACTCAATGC1118130.6344923028802602No Hit
TGAGAATGAGCAGTTT1075020.6100291696335285No Hit
GAGCCTGACCGAGTTT1074810.6099100033616238No Hit
GGAACTACTACCAAGG1058890.6008760650343687No Hit
CTAGAGACTAATCTGC1036030.5879039651498805No Hit
ATGTCCAGAGTCGGTC1030210.5846013570428061No Hit
TCGTATACTGCTAATC1011730.5741147251151885No Hit
TTCGTCCGACCTGTGG994630.5644111858315162No Hit
GAAGTAATGAGTCTGT989440.5614660765401559No Hit
TGCGGTGACCTTAGTG982860.5577322000204739No Hit
ATTCCTAACTGCTACA976170.5539359030726512No Hit
TCACACAGATTGGGAG973280.5522959481878669No Hit
AGGTCGAGAAGGGAGG952330.5404076939192742No Hit
TCCGCGAGAGAACAGC943880.5356126701212024No Hit
ACTGATTCTCGGCTCA943560.535431083421157No Hit
TGGACCTCTAGGGAGG915720.5196330405172134No Hit
TGGCCTGGACCCGAGT907840.5151614680285972No Hit
CCATGATGATTCGGGC892180.5062750688951289No Hit
TTCGATGGAAATCCTG809150.4591589948177426No Hit
GCAATCATGGAACGTG785960.44599963364883266No Hit
ACGAGGATGGAGCTGA783960.4448647167735494No Hit
AAGGTGCGAACAGATA774700.43961005164098765No Hit
TTTCGTAACAGTACGG755700.4288283413257963No Hit
TCCCAGACTGTCTTGA748440.4247085930685178No Hit
TGGTCCTTGGAGAGGG731470.41507882338173907No Hit
TCTCTCTACTCTGGAT717910.4073840869673183No Hit
GCGGTTCGAGTACGTT668510.37935164014782063No Hit
TCCTTCACTTGTCACA633650.35957003901163265No Hit
TCGCCGAGAAGGTAAT632980.35918984185841274No Hit
TTCGATGGACAGGTGA632960.3591784926896599No Hit
TGATATCCTCGATGTT621190.3524995068786177No Hit
AGCCCAGTGAAAGTAC620820.3522895472566903No Hit
CTTTCGGTGCTTTGTT605330.3434996160576211No Hit
AACTCCCTGCTTCACC600430.340719069713177No Hit
AGCTGCTCTTGTCACA595950.33817685591254243No Hit
AACCCTCACGCGGTTC583400.3310552525201397No Hit
CCCTTGCCTCCTACGG579110.328620855822657No Hit
ATGGCCGTGATACGCT575630.326646100459664No Hit
GGGGGGGGGGGGGGGG564080.320091955504903No Hit
TCGGCTTACCTCCTAC557670.31645454691961994No Hit
TGTTTGTTGCAAGTTC527420.29928892918096No Hit
AAACACGCTGCAGTTT526320.2986647248995542No Hit
ACCCTTGTGGGCCATC519430.29475493626420324No Hit
CTAACTGGAACCTTGA511220.29009610249116524No Hit
TGGCCTTTGCGAAAGC499660.2835362829520277No Hit
AAATAGGACTCTATCT493590.2800918102355429No Hit
TACAACGTGAGCAGGT490270.2782078482225726No Hit
CAATGTGTGGTTGACT485250.27535920686561155No Hit
TCAAAGGCTCTTAGTG468620.2659223730476309No Hit
CTTTCGGTGAGCCATT463060.2627673041343433No Hit
TGAACTGTGTGCTGGC456300.2589312850958857No Hit
AGTGTTCGATTCGCCT425550.24148193813840493No Hit
CAGCCGATGTAATAGG416390.2362840188496074No Hit
GAGGTTGGAGGAATAT394750.22400421825904204No Hit
CGACGTTCTTGCTACA365530.20742308271115298No Hit
ACAGCCGACAAACATG363900.2064981254577971No Hit
GGCTTGCGAATACGCT363770.20642435586090369No Hit
GTGCTAATGGGAGTCA361560.20517027271371563No Hit
TTGGGAGGAATCTGTG360080.204330434226006No Hit
TGTACTGGAAATGCTC352580.2000744959436936No Hit
GATAAGAACATTGGTC350760.19904172158718578No Hit
GTAAAGCCTGAAATCA350120.19867854818709513No Hit
AGGGACCCTAATCCTG337520.1915285718728103No Hit
TCTTGAACTCGTACAT323900.18379978795213103No Hit
TAACGAGCTTACTTTC323290.18345363830516961No Hit
GGGTGAAACCGACTAT321340.18234709435176838No Hit
TACGTGTGAAACGCAG315680.17913527959471665No Hit
GGAACATCTTTACGAC315350.1789480183102949No Hit
CGGATGAGAACAGGCA313490.17789254561628143No Hit
TACCACCTGAAACATG310890.17641715367841315No Hit
GGACCACTGGCTAGAG307400.17443672373104377No Hit
AGCTTGTGAACCGTGC305880.17357418690582846No Hit
CATGATGTGCCTAGTA302650.17174129615224593No Hit
ACTTATCTGTCAGGTT299700.17006729376120305No Hit
GGACATTTGGCCGCTA299610.1700162225018153No Hit
AACTAGCACTGCCACG291930.1656581417007274No Hit
ACGTGTTGATCTTTGT282920.16054534117757613No Hit
TGGTAGGGATGTGCTA269890.1531513577351054No Hit
AACTACGACATTGCCA264900.15031974013127355No Hit
TCGGACCGAATGTCCA264610.15015517718435747No Hit
CGTCGTACTTGCGCCA263830.14971255960299698No Hit
CTCGGATCTAGTACGG260980.1480953030557183No Hit
AACGAACCTGACACCC259460.147232766230503No Hit
GTAGATAGAGCTGAGA255160.14479269494864386No Hit
GTCCGAGTGGTCTTGA254540.14444087071730605No Hit
TTTAGGGACTGGGCAA253510.14385638852653515No Hit
AGTTTGCGAGCAGGAT251230.14256258328871219No Hit
TACCACCTGTACTTTC249490.1415752056072157No Hit
CCTTCAGTGCAACGCC249350.14149576142594586No Hit
GGTAGTTTGTCCAGCG244560.13877763550964237No Hit
TGAGGAGTGGTCCCAG243830.13836339085016397No Hit
CTTTAACTGTTCGCCT238380.13527074236501696No Hit
GCCATAATGACCGTTA229210.130067148491843No Hit
AGAACGCGACACAGTT224570.12743414134118575No Hit
CTTACATTGGAAGGCT222250.12611763776585713No Hit
GTCCGTTACGGATGGT216970.1231214572151092No Hit
CAGGCCATGTCGTAGT215290.12216812703987122No Hit
GGGATGTGATGCATGT213710.12127154270839742No Hit
CCGTTACTGTTGGTTG212040.12032388711753586No Hit
TGCAATGACTCCGTAA211970.12028416502690092No Hit
GAGAAGAGATGCTACA205430.11657298684472452No Hit
TCCTCATCTTTCTGGA205410.11656163767597169No Hit
AACTACGACGCTGTTA204950.11630060679465654No Hit
CGATCGGTGCGAGGAT204180.11586366379767246No Hit
GAACCATACTTTGGGT201630.11441664478168627No Hit
GAAAGTCCTTATCTGG200950.11403077304408993No Hit
AATTCTGCTTAGCGAG198820.11282208657191321No Hit
CAAAGACCTGCTTTGA195260.11080193453390894No Hit
ATGGCCGTGGGCCAAA192160.1090428133772198No Hit
CCCACCTCTTGACCTA192150.10903713879284338No Hit
GAAGGACACCCAGCTG191400.10861154496461214No Hit
TGTCTTCCTGAAATCA191380.10860019579585932No Hit
GCTGGACACATTGGTC190280.1079759915144535No Hit
CCTGAGGACTGACTCA188130.10675595587352396No Hit
ACAAGACCTAAGCTAA187740.10653464708284373No Hit
TCGTAGAACCGCACTG186780.10598988698270773No Hit
ATAGCCAACATCTGTG185860.1054678252200774No Hit
TCCGTAAGATTACGCA184060.10444640003232242No Hit
TATGGGTACCCTTTGC182920.10379949741341095No Hit
AGGTGATCTGTTAGTC182000.10327743565078064No Hit
GCGTTTAGAAGTCTAC180950.10268160429125689No Hit
GGCTTAAACTGCATCA179980.1021311696067445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)