FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004949566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13937199
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1539211.1043897701396097No Hit
TGCTGCTACCGTTCTC1422681.0207789958369684No Hit
AAACGACCTCTTCAAG1371920.9843584783427431No Hit
ATGGGTTACAATCGAA1349860.9685303338210209No Hit
TATGCTTTGGACTGTT1324310.9501980993454997No Hit
GCCTCTTGATCTGTGA1251060.8976409104871071No Hit
AAAGCGGACTCGGGCT1243510.8922237531371977No Hit
TCCAAAGTGCTCACGG1168390.8383248312663112No Hit
TAGTGCATGAACCCGC1094520.7853227897513696No Hit
GCAGGTCTGGCTGTTA1049990.7533723239511756No Hit
TGTGGCTCTTACGATC1038420.7450707993765462No Hit
TAACTCACTGGTGTCC1032740.7409953750391308No Hit
GAAGTATGAACAGGTG989150.7097193632666076No Hit
TTCCCATCTCTTTGTT953260.6839681344867071No Hit
TCACCTGACTCAATGC895910.6428192637559383No Hit
GAGCCTGACCGAGTTT886580.6361249487791628No Hit
TGAGAATGAGCAGTTT873030.6264027657207162No Hit
GGAACTACTACCAAGG859710.6168456086477634No Hit
ATGTCCAGAGTCGGTC831670.5967267885031993No Hit
CTAGAGACTAATCTGC830690.5960236343041382No Hit
TTCGTCCGACCTGTGG810700.5816807236518615No Hit
TCGTATACTGCTAATC803620.5766007933157875No Hit
TCACACAGATTGGGAG782350.5613394771790229No Hit
ATTCCTAACTGCTACA780440.5599690439951385No Hit
TCCGCGAGAGAACAGC763330.5476925456829597No Hit
AGGTCGAGAAGGGAGG761630.5464727883988741No Hit
ACTGATTCTCGGCTCA756220.5425910902183431No Hit
TGGACCTCTAGGGAGG736560.5284849559800359No Hit
TGGCCTGGACCCGAGT734350.5268992715107246No Hit
CCATGATGATTCGGGC726000.5209081107330104No Hit
GCAATCATGGAACGTG701380.5032431552423123No Hit
TTCGATGGAAATCCTG637530.4574305066606281No Hit
ACGAGGATGGAGCTGA623410.44729934615987044No Hit
AAGGTGCGAACAGATA611830.43899064654239345No Hit
TTTCGTAACAGTACGG604190.433508913806856No Hit
TCCCAGACTGTCTTGA597680.42883796091309306No Hit
TGGTCCTTGGAGAGGG586310.42067993719541497No Hit
TGCGGTGACCTTAGTG577450.4143228492324749No Hit
TCTCTCTACTCTGGAT577190.4141362981184383No Hit
TCCTTCACTTGTCACA512260.3675487449092174No Hit
TTCGATGGACAGGTGA510220.3660850361683147No Hit
TGATATCCTCGATGTT506690.3635522460431253No Hit
AGCTGCTCTTGTCACA505530.36271994107280814No Hit
AGCCCAGTGAAAGTAC500630.35920417007750266No Hit
TCGCCGAGAAGGTAAT499610.35847231570705135No Hit
AACTCCCTGCTTCACC489520.35123269747386116No Hit
CTTTCGGTGCTTTGTT485700.3484918311060924No Hit
GAAGTAATGAGTCTGT470360.3374853153779321No Hit
CCCTTGCCTCCTACGG464630.333374015826279No Hit
AACCCTCACGCGGTTC464220.333079839069529No Hit
ATGGCCGTGATACGCT458650.3290833402034368No Hit
GGGGGGGGGGGGGGGG457510.3282653853188148No Hit
TCGGCTTACCTCCTAC442300.3173521451476728No Hit
AAACACGCTGCAGTTT425630.3053913487207867No Hit
TGTTTGTTGCAAGTTC424730.30474559486450614No Hit
ACCCTTGTGGGCCATC419060.3006773455699384No Hit
TGGCCTTTGCGAAAGC410430.2944852835924923No Hit
CTAACTGGAACCTTGA404790.29043855942646724No Hit
TACAACGTGAGCAGGT397980.28555235524727746No Hit
CAATGTGTGGTTGACT391960.28123297945304504No Hit
AAATAGGACTCTATCT389160.27922396745572764No Hit
TCAAAGGCTCTTAGTG379740.2724650770933241No Hit
ACAGCCGACAAACATG378220.2713744705804947No Hit
CTTTCGGTGAGCCATT372620.2673564465858599No Hit
GGACATTTGGCCGCTA370650.26594296314489013No Hit
TGAACTGTGTGCTGGC360400.25858854422613897No Hit
AGTGTTCGATTCGCCT345020.24755332832658844No Hit
CAGCCGATGTAATAGG340170.24407343254552077No Hit
GGCTTGCGAATACGCT317580.227865010752878No Hit
GATAAGAACATTGGTC297240.21327097360093658No Hit
GTGCTAATGGGAGTCA294240.21111846074666796No Hit
GTAAAGCCTGAAATCA294230.2111112857038204No Hit
TTGGGAGGAATCTGTG290540.20846369489306996No Hit
CGACGTTCTTGCTACA279700.20068594844631266No Hit
TGTACTGGAAATGCTC277840.19935139047666606No Hit
GGAACATCTTTACGAC273610.19631634735214729No Hit
AGGGACCCTAATCCTG273230.19604369572393995No Hit
GGACCACTGGCTAGAG271010.19445083621178114No Hit
TCTTGAACTCGTACAT258390.18539593213815775No Hit
GGGTGAAACCGACTAT258000.1851161054671028No Hit
TAACGAGCTTACTTTC254300.18246133961350486No Hit
TACGTGTGAAACGCAG253160.18164338472888275No Hit
TACCACCTGAAACATG252100.18088283018704115No Hit
AGCTTGTGAACCGTGC251540.18048102778757769No Hit
CGGATGAGAACAGGCA249540.17904601921806526No Hit
CATGATGTGCCTAGTA243340.17459749265257676No Hit
ACTTATCTGTCAGGTT242470.17397326392483886No Hit
AACTAGCACTGCCACG230990.16573631473583753No Hit
ACGTGTTGATCTTTGT218560.1568177364763178No Hit
TGGTAGGGATGTGCTA216160.1550957261929029No Hit
AACTACGACATTGCCA215650.15472979900767722No Hit
TCGGACCGAATGTCCA213270.15302213880995744No Hit
CGTCGTACTTGCGCCA213180.15295756342432937No Hit
AACGAACCTGACACCC212500.15246966051069516No Hit
GTCCGAGTGGTCTTGA212390.15239073503937198No Hit
GTAGATAGAGCTGAGA210230.15084092578429856No Hit
CTCGGATCTAGTACGG205830.1476839069313712No Hit
AGTTTGCGAGCAGGAT204040.1463995742616576No Hit
TTTAGGGACTGGGCAA202390.14521569219180985No Hit
CCTTCAGTGCAACGCC202160.1450506662063159No Hit
GGTAGTTTGTCCAGCG201310.14444078756427312No Hit
TACCACCTGTACTTTC201200.14436186209294993No Hit
GCCATAATGACCGTTA198430.14237437522417526No Hit
TGAGGAGTGGTCCCAG195890.14055191434089448No Hit
CTTTAACTGTTCGCCT191440.13735902027372932No Hit
AGAACGCGACACAGTT183380.13157593573859425No Hit
CTTACATTGGAAGGCT178930.1283830416714291No Hit
GTCCGTTACGGATGGT176540.12666820643086177No Hit
CAAAGACCTGCTTTGA175510.12592917701756287No Hit
GGGATGTGATGCATGT175160.12567805051789818No Hit
CCGTTACTGTTGGTTG172540.1237981892918369No Hit
CGATCGGTGCGAGGAT171090.1227578080789404No Hit
GAACCATACTTTGGGT170380.1222483800367635No Hit
TGCAATGACTCCGTAA170360.12223402995106836No Hit
CAGGCCATGTCGTAGT169260.12144477523783653No Hit
GAAAGTCCTTATCTGG168970.12123669899525723No Hit
GAGGTTGGAGGAATAT168240.12071292086738519No Hit
GAGAAGAGATGCTACA165510.11875413417000073No Hit
AATTCTGCTTAGCGAG164650.11813708048511039No Hit
AACTACGACGCTGTTA162660.11670924695844553No Hit
GCTGGACACATTGGTC162440.11655139601579916No Hit
TCCTCATCTTTCTGGA162280.11643659533023816No Hit
CCCACCTCTTGACCTA157460.11297822467771321No Hit
GGCTTAAACTGCATCA154170.11061763558086529No Hit
TGTCTTCCTGAAATCA152700.10956290428227365No Hit
ATAGCCAACATCTGTG152420.1093620030825419No Hit
ACAAGACCTAAGCTAA151470.10868037401202349No Hit
TCGTAGAACCGCACTG151010.10835032204103565No Hit
CCTGAGGACTGACTCA150690.10812072066991366No Hit
ATGGCCGTGGGCCAAA150390.1079054693844868No Hit
TATGGGTACCCTTTGC145030.1040596464181935No Hit
TCCGTAAGATTACGCA144430.10362914384733977No Hit
AGGTGATCTGTTAGTC143640.10306231546238236No Hit
ACTGTCCACGTTGGTT141450.10149098107876625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)