FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885970
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT403841.0392257274245555No Hit
TGCTGCTACCGTTCTC394891.0161941548699551No Hit
AAACGACCTCTTCAAG381040.9805531180117191No Hit
ATGGGTTACAATCGAA367330.9452723515621582No Hit
TATGCTTTGGACTGTT360200.926924294320337No Hit
GCAGGTCTGGCTGTTA340010.8749681546692333No Hit
AAAGCGGACTCGGGCT337680.8689722257248511No Hit
TCCAAAGTGCTCACGG320890.8257655102844335No Hit
TAGTGCATGAACCCGC307810.7921059606739115No Hit
GAAGTATGAACAGGTG295890.7614315087352708No Hit
TAACTCACTGGTGTCC291120.7491565812396904No Hit
TGTGGCTCTTACGATC286760.7379367313695164No Hit
TTCCCATCTCTTTGTT255250.6568501558169517No Hit
GGAACTACTACCAAGG245990.6330208416431419No Hit
GCCTCTTGATCTGTGA244210.6284402607328414No Hit
TCACCTGACTCAATGC242350.6236538110175838No Hit
TGAGAATGAGCAGTTT234420.6032470657261894No Hit
ATGTCCAGAGTCGGTC233560.6010339760728981No Hit
CTAGAGACTAATCTGC229210.5898398598033437No Hit
GAAGTAATGAGTCTGT228750.5886561141748392No Hit
TTCGTCCGACCTGTGG225980.581527906803192No Hit
TGCGGTGACCTTAGTG220470.5673486928617565No Hit
TCGTATACTGCTAATC219700.5653672056140423No Hit
TCACACAGATTGGGAG216170.5562832445953005No Hit
ATTCCTAACTGCTACA215260.553941486938911No Hit
TCCGCGAGAGAACAGC210010.5404313466135868No Hit
ACTGATTCTCGGCTCA209800.5398909410005739No Hit
CCATGATGATTCGGGC207230.5332774056413199No Hit
AGGTCGAGAAGGGAGG204560.5264065342758694No Hit
TGGACCTCTAGGGAGG199420.5131794635573614No Hit
TGGCCTGGACCCGAGT195200.5023198840958628No Hit
GCAATCATGGAACGTG194120.499540655228939No Hit
ACGAGGATGGAGCTGA175580.4518305596800799No Hit
TTCGATGGAAATCCTG174630.44938586762121163No Hit
AAGGTGCGAACAGATA168620.433919973648793No Hit
TTTCGTAACAGTACGG164410.423086127787914No Hit
TCCCAGACTGTCTTGA163560.42089877173524237No Hit
TGGTCCTTGGAGAGGG163300.4202296981191312No Hit
GCGGTTCGAGTACGTT160260.412406683530753No Hit
TCTCTCTACTCTGGAT157530.40538141056158433No Hit
TCCTTCACTTGTCACA145950.3755819010440122No Hit
TCGCCGAGAAGGTAAT139040.3577999830158236No Hit
TTCGATGGACAGGTGA137880.35481488534394245No Hit
AGCTGCTCTTGTCACA136480.35121218125718934No Hit
TGATATCCTCGATGTT136320.3508004436472747No Hit
AACTCCCTGCTTCACC132070.339863663383917No Hit
CTTTCGGTGCTTTGTT131790.3391431225665664No Hit
AGCCCAGTGAAAGTAC129690.33373906643643675No Hit
AACCCTCACGCGGTTC122920.31631741881692343No Hit
ATGGCCGTGATACGCT121160.31178830510786243No Hit
CCCTTGCCTCCTACGG120970.31129936669608876No Hit
TCGGCTTACCTCCTAC120330.30965241625643014No Hit
TGTTTGTTGCAAGTTC117760.30303888089717623No Hit
CTAACTGGAACCTTGA117640.3027300776897403No Hit
AAACACGCTGCAGTTT114970.2958592063242897No Hit
TACAACGTGAGCAGGT110070.2832497420206538No Hit
AAATAGGACTCTATCT109540.28188586118781156No Hit
TGGCCTTTGCGAAAGC109010.2805219803549693No Hit
CAATGTGTGGTTGACT107230.2759413994446689No Hit
TCAAAGGCTCTTAGTG106440.27390844499571537No Hit
GGGGGGGGGGGGGGGG106280.2734967073858007No Hit
ACAGCCGACAAACATG104720.26948226568913297No Hit
CTTTCGGTGAGCCATT101970.26240552551872504No Hit
GGACATTTGGCCGCTA101750.2618393863050924No Hit
ACCCTTGTGGGCCATC101280.26062990707596817No Hit
TGAACTGTGTGCTGGC98670.25391343731423555No Hit
CAGCCGATGTAATAGG96570.2485093811841059No Hit
AGTGTTCGATTCGCCT94320.24271932104468125No Hit
GAGGTTGGAGGAATAT93090.23955408816846244No Hit
GATAAGAACATTGGTC92130.23708366250897459No Hit
GGCTTGCGAATACGCT85750.22066562531362827No Hit
GTGCTAATGGGAGTCA85060.21889000687087137No Hit
CGACGTTCTTGCTACA83350.21448956116490864No Hit
GTAAAGCCTGAAATCA82410.21207060270666012No Hit
TTGGGAGGAATCTGTG81540.20983177945274925No Hit
TGTACTGGAAATGCTC79700.20509679693873087No Hit
GGAACATCTTTACGAC77120.19845752797885727No Hit
TCTTGAACTCGTACAT75190.1934909430592619No Hit
AGGGACCCTAATCCTG73310.18865302614276486No Hit
GGGTGAAACCGACTAT71710.18453565004361844No Hit
TAACGAGCTTACTTTC71100.1829659004058189No Hit
CGGATGAGAACAGGCA70620.18173068757607497No Hit
TACCACCTGAAACATG70230.180727077151908No Hit
TACGTGTGAAACGCAG69990.18010947073703604No Hit
AACTAGCACTGCCACG68520.1763266314459453No Hit
CATGATGTGCCTAGTA68000.1749884842137227No Hit
AGCTTGTGAACCGTGC66990.17238939055113653No Hit
ACTTATCTGTCAGGTT66330.17069097291023863No Hit
ACGTGTTGATCTTTGT64060.16484944556957465No Hit
TCGGACCGAATGTCCA61700.15877631582333368No Hit
AACTACGACATTGCCA60930.15679482857561947No Hit
TGGTAGGGATGTGCTA60310.15519934533720023No Hit
GTAGATAGAGCTGAGA60140.15476187412666592No Hit
CTCGGATCTAGTACGG58450.15041289562194252No Hit
AACGAACCTGACACCC58120.14956368680149357No Hit
CGTCGTACTTGCGCCA57960.14915194919157893No Hit
GGTAGTTTGTCCAGCG56770.1460896507178388No Hit
TTTAGGGACTGGGCAA56550.14552351150420617No Hit
TGAGGAGTGGTCCCAG55480.142770016237902No Hit
TACCACCTGTACTTTC55400.14256414743294465No Hit
CCTTCAGTGCAACGCC55320.14235827862798736No Hit
AGTTTGCGAGCAGGAT54960.1414318690056794No Hit
CTTTAACTGTTCGCCT52860.13602781287554974No Hit
CGATCGGTGCGAGGAT52560.1352558048569598No Hit
CAGGCCATGTCGTAGT51160.13165310077020667No Hit
GAGAAGAGATGCTACA50450.12982601512621045No Hit
AGAACGCGACACAGTT49300.12686665105494896No Hit
GCCATAATGACCGTTA49270.12678945025308996No Hit
CTTACATTGGAAGGCT49110.12637771264317532No Hit
GAAAGTCCTTATCTGG48720.12537410221900838No Hit
GGGATGTGATGCATGT48580.12501383181033307No Hit
GAAGGACACCCAGCTG47640.12259487335208455No Hit
CAAAGACCTGCTTTGA47490.12220886934278957No Hit
TGCAATGACTCCGTAA47420.12202873413845192No Hit
CCGTTACTGTTGGTTG46240.11899216926533143No Hit
AATTCTGCTTAGCGAG45980.11832309564922014No Hit
AACTACGACGCTGTTA45890.11809149324364315No Hit
TCCTCATCTTTCTGGA45370.11675334601142058No Hit
GGCTTAAACTGCATCA45080.11600707159345029No Hit
GCTGGACACATTGGTC44510.11454025635812938No Hit
TGTCTTCCTGAAATCA43240.11127208907943191No Hit
CCTGAGGACTGACTCA42800.11013981065216664No Hit
ACAAGACCTAAGCTAA42520.10941926983481602No Hit
GCGTTTAGAAGTCTAC42500.10936780263357669No Hit
CCCACCTCTTGACCTA42490.10934206903295703No Hit
ATAGCCAACATCTGTG41890.1077980529957771No Hit
TCCGTAAGATTACGCA41650.10718044658090514No Hit
ATGGCCGTGGGCCAAA41190.10599670095240055No Hit
TCGTAGAACCGCACTG40990.10548202894000726No Hit
AGGTGATCTGTTAGTC40600.10447841851584032No Hit
GCGTTAATGGTACGTT40260.1036034760947717No Hit
GGACCACTGGCTAGAG40130.10326893928671604No Hit
TATGGGTACCCTTTGC39940.10278000087494243No Hit
ACTGTCCACGTTGGTT39250.10100438243218553No Hit
ATCTCTTGACGGTACC38890.10007797280987757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)