FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376034
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC327281196.94247747504913Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC254350.7533988105570026No Hit
TGTAGATA182770.5413748795183935No Hit
AGTAGATC62900.1863132894988617No Hit
TGTAGAAC61780.1829957873647007Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC59710.17686433252745679No Hit
TGTACATC56410.16708954945358964No Hit
TCTAGATC39680.11753436132456012ABI Dynabead EcoP Oligo (100% over 8bp)
TGTAGATG37410.11081049539193029No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)