FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778089
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT493711.7771568873423422No Hit
GAAGCAGTGGAACCTT153500.5525380936319895No Hit
GTTCTTCCTAACGTAA140850.5070031953619917No Hit
GATACGTACCGCTCGA133650.4810860991134553No Hit
CGCTTAAGATAATAGG115110.4143495762734743No Hit
TGTCCCATGAGACAAT112830.4061424957947712No Hit
CATGGAGGATCGACAT111910.4028308668296804No Hit
AAAGCATGATCTTTGT104430.37590588350481213No Hit
TGAGCGCACAGGACAC97640.3514646219037619No Hit
TGTCGAAACTTCGCAA92510.33299869082667977No Hit
GGGGGGGGGGGGGGGG90230.3247916103479766No Hit
TAATTGCCTGTCTGGC90220.3247556143809648No Hit
GGAAGGACTAGGTAAT76580.27565711537679316No Hit
AATGATGGAAAGCTAA73270.26374245029586885No Hit
CATGTCAGAGGTTTGT72550.2611507406710152No Hit
TCGACCTTGTTTCGCC69350.2496320312272213No Hit
ATGGGTACTTGGCTGC57730.20780471755944466No Hit
GCTATGATGACCTGCG53630.1930463710845837No Hit
CAGCTCACTGTTTACG52290.18822291150499498No Hit
TTGCGATGACATTCAG51960.18703504459360373No Hit
AAACGACCTAGGGAGG51030.18368741966150112No Hit
TGTTCCTCTTTATGGC50040.18012381892732737No Hit
AGCCTACCTTAACTCG49860.17947589152111398No Hit
CACAGCTCTGGACTGA46810.16849712158249788No Hit
CTGCCTTGACCATAAC46620.16781319820927265No Hit
CAATGTGACCAACGCC46570.16763321837421336No Hit
CCTGTTGACCCTAGTA46350.1668413070999525No Hit
CATGCAAACGAAGGCT44660.16075798867494887No Hit
AGCTTGTGACTCACTC44400.1598220935326406No Hit
ATGGGTACTTTCGGGC44060.1585982306542375No Hit
CATGATGTGTCATGTG43970.1582742669511308No Hit
TAACCAGACATCACAC43300.15586253716133647No Hit
CTATTCAACCGAACGA42950.15460267831592148No Hit
GTGAGATCTCGATTAC41650.1499232026043802No Hit
TGGGCCATGCGTTCTC41570.14963523486828534No Hit
AGAACGCGAGGAATAT41340.14880732762701268No Hit
TTGTTTGTGATACGCT41170.14819539618781113No Hit
ATGCCGCGATGCCACG40700.1465035857382539No Hit
CCGCTATTGTTCGCAA40660.14635960187020647No Hit
ATTAGCGTGGTCGGTC40130.1444518156185781No Hit
CACTATACTACGCCAC39940.14376789224535283No Hit
CATTAGTCTATTCTGG39780.14319195677316313No Hit
GGGCTGACTAATACAG39040.1405282552142858No Hit
GCATCTCTGAGCCTAT38820.13973634394002496No Hit
GGGTCGTACCGCTCGA38610.138980428632776No Hit
AGCTCGGACCTTAAGC38200.13750459398528989No Hit
ATAGGAAACAGACTTC37480.13491288436043625No Hit
GCAGATACTCGGCTCA37180.13383300535008058No Hit
AACCAACACCGGAATG36520.13145727152729808No Hit
TTGACGTGACCTCAAA36400.1310253199231558No Hit
CCCAATGCTGGAATGC36300.13066536025303724No Hit
TGGAGTTACAAGTCTC36280.13059336831901353No Hit
ATAGCCAACATGGCCC35820.12893755383646816No Hit
GGCTTGCGAGTACGTT35520.12785767482611246No Hit
AACGTCCCTTACAGAA35440.12756970709001764No Hit
CTGCCATTGCACAGTT35220.1267777958157568No Hit
TTGTAACCTCATCCTC35150.1265258240466738No Hit
CTGATTACTGCTTTGA35020.1260578764755197No Hit
CCTACGGGATGGAACG34570.12443805795998616No Hit
GGTAGCCTGTGCCACG34350.12364614668572532No Hit
GATACCATGTCTGGAT34080.12267425557640523No Hit
CGATCAAACCGATCGC33920.12209832010421554No Hit
TGTTTGTTGCGCTCGA33840.12181035236812068No Hit
CGCTTACTGGCCACGT33570.12083846125880056No Hit
ATCTTACTGCGCACTG33120.11921864274326704No Hit
CATGTTACTAACGTGG32920.11849872340302993No Hit
TCAGGCTCTTAAAGCA32860.11828274760095879No Hit
CGCTGAGTGTTAGCTG32850.11824675163394695No Hit
CTCGGATCTTTCTGAG32790.11803077583187581No Hit
GGGATCAGATGAGTAA32780.11799477986486394No Hit
TCCAAAGTGAAGGCCA32250.11608699361323557No Hit
CGCTTCCCTGCGCTGT32090.11551105814104588No Hit
TGGGTGCACTTCTGAG31860.11468315089977318No Hit
TTCTAACACTACGTTG31530.11349528398838195No Hit
CCTTCAGTGTAGCTTG31280.11259538481308554No Hit
CCTCCCTTGTTTCGCC31240.11245140094503811No Hit
TGAGATAGACTCATCC30240.10885180424385253No Hit
GCATAGTACGCTCCTT30230.10881580827684066No Hit
AACCACTGAAAACATG30110.10838385667269838No Hit
AGCCGGTACGCGCAAA30080.10827586877166283No Hit
CTTAGGCCTTATGACC30050.10816788087062724No Hit
ATTGAGAGATGATCAC29980.10791590910154426No Hit
TACAACCGATTCCATT29830.10737596959636642No Hit
GTCCTGACTTTACGAC29810.1073039776623427No Hit
AGTGGTACTCAACGCC29780.10719598976130715No Hit
ACCTGCTTGCCAAACT29700.1069080220252123No Hit
AACAGCTACTCGAGAG29700.1069080220252123No Hit
AGAACAATGAGTCGGA29680.10683603009118858No Hit
GTGTACTGAATCACAC29540.1063320865530226No Hit
ATCGCAATGTAACTCG29420.10590013494888033No Hit
TATCTCCCTGGAATTA29330.10557617124577362No Hit
CATGTCAGAACAGGTG29160.10496423980657207No Hit
ACCTCCATGGAATTGA28980.10431631240035866No Hit
TTCACGCTGTGGGCAA28900.10402834466426382No Hit
CGAGTCTCTTACTTTC28830.10377637289518084No Hit
TACGCCTTGGGTTGAG28720.1033804172580504No Hit
TTCTATGACCAACGCC28490.10255251001677773No Hit
TGGTCCTTGTTTCTGT28440.10237253018171844No Hit
GCATGGGCTATGTGGG28340.10201257051159987No Hit
ATTCCTAACATTGCCA28280.10179659470952875No Hit
GTAAGAAACGACAAAT27950.10060872779813751No Hit
AGAACATGAAAGCTAA27900.10042874796307821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)