FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300756
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC148950.6473958994347945No Hit
CTCCTAGTGAACGTAA119360.5187859990368383No Hit
TAGCTTTACCCTATCC104530.45432892492728477No Hit
CGACCAGACGCGCTGT85430.3713127337275226No Hit
CCAGGACTGTAACTCG81930.35610034266997453No Hit
ATCTGTACTGGGACTA74970.32584941645267906No Hit
GTTCTCGACCGTAGAG72100.31337525578548964No Hit
CTTCCTTTGGCTCAGC70110.30472592486991235No Hit
CGATCAAACCCGGCTA68050.29577234613318404No Hit
GGGGGGGGGGGGGGGG60310.2621312299087778No Hit
TGCTGCTACTTTCTTG58870.25587241758795803No Hit
ACAGCCGACTTGCGTA56200.2442675364097714No Hit
NNNNNNNNNNNNNNNN51320.2230571168781044No Hit
AACTTACCTCACCTAA50230.21831954366303943No Hit
GGCAACAGAATAGCAA50040.21749372814848683No Hit
CGCGTTGGAGCGGTTC47290.2055411351746991No Hit
ATCGAAAGATTTCCGC46120.20045585016403306No Hit
GTAACTGTGTGAAAGT43320.18828593731799462No Hit
CCCTGAACTAGCTTTG43140.18750358577789214No Hit
AGTGGAGTGAGTTGAA42680.18550424295318582No Hit
CAATTCGGATAACGGG42670.1854607789787357No Hit
GGGTAGAACCAGTCTA38790.16859675689208242No Hit
TTCCTAAACTAATGCC37940.16490231906382075No Hit
AAGAACCCTTAACCCA37130.16138173713335965No Hit
GTGAAGCGATCCCAGG36920.16046899366990675No Hit
TTTAGTCTGAGCAATA36510.15868697071745114No Hit
CGCGATCGATATCTGG36370.1580784750751492No Hit
TGGAACACTCCTAGTA35440.15403632545128645No Hit
TGCAGATACTGCCACG34690.15077652736752617No Hit
TCCGGGAACGTTCTCC33660.14629973799916202No Hit
TGATAGAGAGACTGTT33090.1438222914555042No Hit
CTTTAACTGTCCGTAA33050.14364843555770362No Hit
AGTCCTGCTCGAACGA32970.14330072376210257No Hit
CACATCCTGAGCCTAT32400.14082327721844473No Hit
AGGTGTGGATCATGTG32260.14021478157614278No Hit
TCTCAGAGATGCATGT31490.13686805554348222No Hit
TGCAGTATGACACCGC31450.1366941996456817No Hit
GCAACTTGAAGGTGCA31310.13608570400337974No Hit
CATAGGTACAAGGTGC31170.13547720836107785No Hit
CTATTGGGACTTGTGA30760.1336951854086222No Hit
TGGCAATTGAGTGGTG30450.13234780220066797No Hit
CTTAGATCTTGTCACA30430.13226087425176766No Hit
TACGTTTACTGCACCG30290.13165237860946577No Hit
AGAGTACCTTAATCGC29780.12943571591250874No Hit
TCAAAGCACTCTGCAA29740.1292618600147082No Hit
ATTTCTGACGGGTAGT29680.12900107616800738No Hit
CGAGTCTCTGCTACGC29040.1262193818031986No Hit
ATGTACGTGTACTTTC28970.12591513398204765No Hit
TGAGGGATGTACGTTG28910.12565435013534682No Hit
TCTATGGGATGCTCAA28750.12495892654414462No Hit
GAGTGTTACACCTGCG28740.12491546256969449No Hit
GGTCGCATGGTACTAC28380.12335075948948955No Hit
GGTCTAAACTCCTACC28220.12265533589828734No Hit
GGGTGTGCTCGCAAAG27690.12035174525243007No Hit
TGAACTGTGAACGTAA27550.11974324961012817No Hit
GGGATGTGACTGGAAG27480.11943900178897719No Hit
TTCTTAGACGTCCCGA27450.11930860986562677No Hit
TACTGTTTGCTTAAGC26880.11683116332196895No Hit
CATTCTATGCCTAGCG26530.11530992421621415No Hit
TCCATTTGAAATCTGC26240.11404946895716017No Hit
CTACAACGAATCTGTG26210.11391907703380975No Hit
GTAATCTCTACACAAA26020.11309326151925714No Hit
CACCAGGTGCGCCACA25990.11296286959590675No Hit
AGACTACTGGCCCTAG25860.11239783792805495No Hit
GCATGATACTTGCGTA25790.11209359010690399No Hit
TTGCGGTCTTTTGGGT25260.10978999946104671No Hit
CTCAATTTGGAATCTC25070.10896418394649411No Hit
TGCTAGTGAAGGCGTA25040.10883379202314371No Hit
CTAGCTCACGAATAGT25040.10883379202314371No Hit
TCCGGGTGAACACCTA24940.10839915227864232No Hit
CTTTCGTCTCGAACGA24940.10839915227864232No Hit
TCGGACCGATAGGCGA24860.10805144048304123No Hit
CGAGTCTCTTGGAACG24810.10783412061079055No Hit
AGGCGATGACCTGCGA24670.10722562496848861No Hit
CACATTACTGATGGAC24650.10713869701958834No Hit
TGTAAGCACGCGCTGT24060.10457432252703025No Hit
TGCGCAGACATCAGGG23750.10322693931907598No Hit
ACGAGGATGAACTAGA23630.10270537162567434No Hit
AGCCAATTGTTATGGC23550.10235765983007325No Hit
ATGTCCAGACCGCTAG23530.10227073188117297No Hit
ACCAAGTTGAAGCTAA23310.10131452444326995No Hit
TAGTACCGAACGAGCA23300.10127106046881981No Hit
GGCTGACTGAAACTGA23190.10079295674986831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)