FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303381
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT345611.0462311189656899No Hit
TGCTGCTACCGTTCTC333871.0106917730652323No Hit
AAACGACCTCTTCAAG328560.9946173329688582No Hit
ATGGGTTACAATCGAA319050.9658286464685726No Hit
TATGCTTTGGACTGTT313160.947998429487849No Hit
AAAGCGGACTCGGGCT289860.8774646339613869No Hit
GCCTCTTGATCTGTGA287240.8695333659665658No Hit
GCAGGTCTGGCTGTTA280520.8491905717203072No Hit
TCCAAAGTGCTCACGG273000.8264260162542558No Hit
TAGTGCATGAACCCGC262860.7957301927933835No Hit
GAAGTATGAACAGGTG245460.7430568862628926No Hit
TAACTCACTGGTGTCC244930.7414524694547798No Hit
TGTGGCTCTTACGATC244800.7410589332565635No Hit
TTCCCATCTCTTTGTT221760.671312210126534No Hit
TCACCTGACTCAATGC215990.6538452573287792No Hit
GGAACTACTACCAAGG204680.6196076080839601No Hit
GAGCCTGACCGAGTTT203630.6164290464829821No Hit
TGAGAATGAGCAGTTT203550.6161868703610028No Hit
ATGTCCAGAGTCGGTC195460.5916968100258493No Hit
TTCGTCCGACCTGTGG193550.5859148551135942No Hit
TCGTATACTGCTAATC191240.5789220195914428No Hit
GAAGTAATGAGTCTGT189540.5737757769993834No Hit
CTAGAGACTAATCTGC188740.5713540157795907No Hit
TCACACAGATTGGGAG188040.569234974712272No Hit
TGCGGTGACCTTAGTG186950.5659353250503045No Hit
TCCGCGAGAGAACAGC183740.5562180081558864No Hit
ATTCCTAACTGCTACA181130.5483170121763127No Hit
AGGTCGAGAAGGGAGG177550.5374796307177404No Hit
TGGACCTCTAGGGAGG176000.5327874683543921No Hit
ACTGATTCTCGGCTCA175490.5312435955767743No Hit
TGGCCTGGACCCGAGT172860.5232820555667057No Hit
CCATGATGATTCGGGC167570.5072681595008266No Hit
GCAATCATGGAACGTG160250.48510904433972346No Hit
TTCGATGGAAATCCTG150920.4568652541138912No Hit
ACGAGGATGGAGCTGA150890.45677443806814894No Hit
AAGGTGCGAACAGATA144900.4386415009349512No Hit
TCCCAGACTGTCTTGA142220.43052860084864564No Hit
TTTCGTAACAGTACGG142080.430104792635182No Hit
TGGTCCTTGGAGAGGG138500.4192674111766097No Hit
TCTCTCTACTCTGGAT137710.41687592197206436No Hit
GCGGTTCGAGTACGTT121900.36901586586591134No Hit
TCGCCGAGAAGGTAAT120290.3641420714110785No Hit
TCCTTCACTTGTCACA120270.36408152738058375No Hit
TTCGATGGACAGGTGA119360.3613267739930695No Hit
TGATATCCTCGATGTT118720.3593893650172354No Hit
AGCTGCTCTTGTCACA116320.35212408135785733No Hit
AGCCCAGTGAAAGTAC116120.35151864105290914No Hit
AACTCCCTGCTTCACC115080.34837035146717865No Hit
CTTTCGGTGCTTTGTT111550.3376843300848434No Hit
AACCCTCACGCGGTTC110110.3333251598892165No Hit
CCCTTGCCTCCTACGG108280.32778538109894073No Hit
ATGGCCGTGATACGCT107960.32681667661102365No Hit
TCGGCTTACCTCCTAC103230.3124980133989994No Hit
TGTTTGTTGCAAGTTC101160.30623170624278584No Hit
AAACACGCTGCAGTTT99300.30060111140676776No Hit
TGGCCTTTGCGAAAGC96970.2935477318541216No Hit
ACCCTTGTGGGCCATC96840.2931541956559053No Hit
CTAACTGGAACCTTGA94340.2855861918440531No Hit
TACAACGTGAGCAGGT93740.2837698709292086No Hit
GGGGGGGGGGGGGGGG93360.2826195343498071No Hit
AAATAGGACTCTATCT93230.2822259981515907No Hit
CAATGTGTGGTTGACT93040.28165082986189No Hit
TCAAAGGCTCTTAGTG91070.2756872428581505No Hit
ACAGCCGACAAACATG87530.2649709494605678No Hit
CTTTCGGTGAGCCATT86530.261943747935827No Hit
TGAACTGTGTGCTGGC84330.25528390458139705No Hit
AGTGTTCGATTCGCCT82280.2490781414556783No Hit
GGACATTTGGCCGCTA81350.24626284403766927No Hit
CAGCCGATGTAATAGG78050.23627307900602443No Hit
GAGGTTGGAGGAATAT76110.23040030804802716No Hit
CGACGTTCTTGCTACA71530.216535725064714No Hit
TGTACTGGAAATGCTC67490.2043058309047609No Hit
TTGGGAGGAATCTGTG67330.20382147866080236No Hit
GTGCTAATGGGAGTCA66950.20267114208140083No Hit
GGAACATCTTTACGAC64110.1940738897511368No Hit
AGGGACCCTAATCCTG62890.19038070389095293No Hit
GGACCACTGGCTAGAG62720.18986607963174698No Hit
TCTTGAACTCGTACAT62370.1888065590980877No Hit
TACCACCTGAAACATG61640.18659670198502687No Hit
GGGTGAAACCGACTAT61390.18583990160384165No Hit
TAACGAGCTTACTTTC60060.18181372357593628No Hit
TACGTGTGAAACGCAG60010.18166236349969925No Hit
CGGATGAGAACAGGCA59760.18090556311851402No Hit
ACTTATCTGTCAGGTT57800.17497224813002193No Hit
AGCTTGTGAACCGTGC57570.17427599177933156No Hit
CATGATGTGCCTAGTA54290.16434677077818152No Hit
TCGGACCGAATGTCCA53240.16116820917720362No Hit
AACTAGCACTGCCACG53140.1608654890247295No Hit
ACGTGTTGATCTTTGT52230.15811073563721534No Hit
GGCTTGCGAATACGCT51850.15696039905781378No Hit
TGGTAGGGATGTGCTA51340.15541652628019595No Hit
AACGAACCTGACACCC50350.15241959677070252No Hit
GTCCGAGTGGTCTTGA50330.1523590527402077No Hit
CGTCGTACTTGCGCCA50230.1520563325877336No Hit
GTAGATAGAGCTGAGA49910.15108762809981652No Hit
AACTACGACATTGCCA49070.1485447788190342No Hit
CTCGGATCTAGTACGG48460.14669818588894226No Hit
GGTAGTTTGTCCAGCG48430.14660736984320005No Hit
AGTTTGCGAGCAGGAT47630.14418560862340735No Hit
TACCACCTGTACTTTC47580.1440342485471703No Hit
TTTAGGGACTGGGCAA47450.143640712348954No Hit
CCTTCAGTGCAACGCC47140.1427022798762843No Hit
TGAGGAGTGGTCCCAG46850.1418243914341095No Hit
GCCATAATGACCGTTA45340.13725331713175076No Hit
CTTACATTGGAAGGCT44160.13368121933255656No Hit
CTTTAACTGTTCGCCT43490.13165299431098018No Hit
GTCCGTTACGGATGGT42630.12904960099970303No Hit
AGAACGCGACACAGTT42390.12832307263376522No Hit
GGGATGTGATGCATGT41770.1264462076884259No Hit
CGATCGGTGCGAGGAT41680.12617375955119922No Hit
CAGGCCATGTCGTAGT41390.12529587110902435No Hit
GAGAAGAGATGCTACA41130.12450879871259174No Hit
GATAAGAACATTGGTC41040.12423635057536506No Hit
CAAAGACCTGCTTTGA40990.12408499049912801No Hit
TGCAATGACTCCGTAA40540.12272274981299461No Hit
CCGTTACTGTTGGTTG39650.12002854045597526No Hit
GAAAGTCCTTATCTGG39640.11999826844072785No Hit
GAACCATACTTTGGGT39410.11930201209003745No Hit
TCCTCATCTTTCTGGA39080.11830303558687297No Hit
AACTACGACGCTGTTA38280.11588127436708029No Hit
AATTCTGCTTAGCGAG38170.11554828219935878No Hit
CCCACCTCTTGACCTA37190.11258162470511274No Hit
TGTCTTCCTGAAATCA37120.11236972059838087No Hit
TCGTAGAACCGCACTG36950.11185509633917493No Hit
GAAGGACACCCAGCTG36750.11124965603422675No Hit
TCCGTAAGATTACGCA36250.10973605527185631No Hit
CCTGAGGACTGACTCA36230.10967551124136149No Hit
ATGGCCGTGGGCCAAA35860.10855544667720737No Hit
AGGTGATCTGTTAGTC34900.10564933321345615No Hit
TATGGGTACCCTTTGC34390.1041054604358383No Hit
GTAAAGCCTGAAATCA33960.10280376378019974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)