FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535919
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGTCC437916096.5440520432574No Hit
CCCAGTCC458881.011658276966586No Hit
ACCAGTCA280320.6180004537118057No Hit
ACCAGACC105080.2316619851456783No Hit
ACAAGTCC96970.2137824771562279No Hit
ACCACTCC96320.2123494709671844No Hit
ACCCGTCC63900.1408755315075071TruSeq Adapter, Index 16 (100% over 8bp)
ACCAGTAC53900.1188292824452994No Hit
ACCAGTCG50950.11232563897194812No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)