FastQCFastQC Report
Wed 8 May 2024
EGAF00004949639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3722609
Total Bases29.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT356034795.64117531548439No Hit
CTTTCCTA685681.841933976950037No Hit
CTTTCCAT108620.29178460590408506No Hit
CTTTACTT100840.27088528502456205No Hit
CTTTCATT94350.25345127570475434No Hit
CATTCCTT75290.20225062583795397TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT74800.20093434470286833No Hit
ATTTCCTT67510.18135130495843102No Hit
CGTTCCTT55550.1492233001102184No Hit
CTTTCCGT52750.14170169362401477No Hit
CTTACCTT49240.13227282263595236No Hit
CTGTCCTT47760.1282971163503876No Hit
CTTTCGTT44630.11988903481402426No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)