FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3968826
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT384865496.97210207754132No Hit
CTAGCTGA226660.571100874666715No Hit
CTAGCAGT84790.21364000336623473No Hit
CTAGCTGG74310.18723420981418687No Hit
CTAGATGT70510.17765959001478018No Hit
CTAGCGGT68820.17340140384083355No Hit
CTAGCTAT68210.17186442539934985RNA PCR Primer, Index 38 (100% over 8bp)
CAAGCTGT67250.169445574081605No Hit
CTCGCTGT65120.16407874772035863No Hit
CTAGCTCT60260.15183331292427535No Hit
CTACCTGT57910.1459121664693791No Hit
ATAGCTGT51940.13086993483715337No Hit
CTTGCTGT49500.12472202107121853No Hit
CTAGCTTT42330.10665622529181173No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)