FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240166
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT587421.8129318065802802No Hit
GAAGCAGTGGAACCTT197040.6081169915368534No Hit
GTTCTTCCTAACGTAA164320.5071345110096211No Hit
CGCTTAAGATAATAGG137560.424546149796029No Hit
CATGGAGGATCGACAT136080.419978482583917No Hit
TGTCCCATGAGACAAT132600.4092382921121943No Hit
AAAGCATGATCTTTGT123520.38121503651356137No Hit
TGAGCGCACAGGACAC120790.37278954226419264No Hit
GATACGTACCGCTCGA118380.3653516517363617No Hit
TGTCGAAACTTCGCAA111420.34387127079291613No Hit
TAATTGCCTGTCTGGC111310.34353178201363754No Hit
GGAAGGACTAGGTAAT93110.2873618203511795No Hit
GGGGGGGGGGGGGGGG92620.2858495521525749No Hit
AATGATGGAAAGCTAA88960.27455383458748717No Hit
CATGTCAGAGGTTTGT87800.27097377109691295No Hit
TCGACCTTGTTTCGCC86210.2660666151055224No Hit
GTGAGATCTCGATTAC69690.2150815729811374No Hit
ATGGGTACTTGGCTGC68570.21162495995575537No Hit
AAACGACCTAGGGAGG65460.20202668628706058No Hit
TTGCGATGACATTCAG61630.19020630424490598No Hit
AGCCTACCTTAACTCG60980.18820023418553247No Hit
TGTTCCTCTTTATGGC60360.18628675197505312No Hit
GCTATGATGACCTGCG59680.18418809406678546No Hit
CAGCTCACTGTTTACG57870.17860196051683772No Hit
CTGCCTTGACCATAAC57320.17690451662044474No Hit
CAATGTGACCAACGCC56450.17421946900251406No Hit
CCTGTTGACCCTAGTA56010.17286151388539972No Hit
CATGATGTGTCATGTG54680.16875678591775853No Hit
TAACCAGACATCACAC54000.16665812800949087No Hit
CACAGCTCTGGACTGA53880.16628777661391422No Hit
CATGCAAACGAAGGCT53660.16560879905535705No Hit
ATGGGTACTTTCGGGC52960.16344841591449327No Hit
ATGCCGCGATGCCACG51370.1585412599231027No Hit
CTATTCAACCGAACGA51290.15829435899271826No Hit
TTGTTTGTGATACGCT50800.15678209079411362No Hit
CATTAGTCTATTCTGG50330.1553315478281051No Hit
TGGGCCATGCGTTCTC50180.1548686085836343No Hit
AGAACGCGAGGAATAT49910.15403531794358685No Hit
ATTAGCGTGGTCGGTC47660.1470912292765247No Hit
AGCTTGTGACTCACTC47060.1452394722986415No Hit
CCGCTATTGTTCGCAA46980.14499257136825705No Hit
CACTATACTACGCCAC45990.14193717235474973No Hit
CCCAATGCTGGAATGC45030.13897436119013654No Hit
GGGCTGACTAATACAG44850.13841883409677158No Hit
AACCAACACCGGAATG44560.137523818224128No Hit
GCAGATACTCGGCTCA43930.13557947339735063No Hit
ATAGGAAACAGACTTC43700.13486963322249537No Hit
GGTAGCCTGTGCCACG43140.1331413267098044No Hit
AACGTCCCTTACAGAA42940.1325240743838433No Hit
TTGACGTGACCTCAAA42900.1324006239186511No Hit
TCAGGCTCTTAAAGCA42710.13181423420898805No Hit
TGGAGTTACAAGTCTC42700.13178337159269No Hit
CTGCCATTGCACAGTT42570.1313821575808153No Hit
ATAGCCAACATGGCCC42340.13067231740596005No Hit
CCTACGGGATGGAACG42260.13042541647557562No Hit
AGCTCGGACCTTAAGC42090.1299007519985087No Hit
TTGTAACCTCATCCTC41500.12807985763692353No Hit
GCATCTCTGAGCCTAT41110.12687621560129944No Hit
CTGATTACTGCTTTGA40970.12644413897312667No Hit
TGTTTGTTGCGCTCGA40500.12499359600711817No Hit
TCCAAAGTGAAGGCCA40140.12388254182038821No Hit
CGCTGAGTGTTAGCTG39960.12332701472702326No Hit
GGGATCAGATGAGTAA39790.12280235024995632No Hit
CGCTTACTGGCCACGT39640.12233941100548552No Hit
CGCTTCCCTGCGCTGT39360.12147525774914002No Hit
TTCTAACACTACGTTG39070.12058024187649645No Hit
CGATCAAACCGATCGC39000.12036420356241007No Hit
TGGGTGCACTTCTGAG38740.11956177553866067No Hit
ATCTTACTGCGCACTG38660.11931487460827624No Hit
CATGTTACTAACGTGG38490.11879021013120933No Hit
CCTTCAGTGTAGCTTG37980.11721621670000859No Hit
CTCGGATCTTTCTGAG37720.11641378867625918No Hit
ATTGAGAGATGATCAC37180.11474720739616427No Hit
CCTCCCTTGTTTCGCC37050.11434599338428957No Hit
CTTAGGCCTTATGACC36810.11360529059313627No Hit
ACCTGCTTGCCAAACT36690.11323493919755963No Hit
TACGCCTTGGGTTGAG36590.11292631303457909No Hit
TACAACCGATTCCATT36440.11246337379010828No Hit
GATACCATGTCTGGAT36360.11221647285972385No Hit
AGCCGGTACGCGCAAA36300.11203129716193554No Hit
CGAGTCTCTTACTTTC36200.11172267099895498No Hit
AACCACTGAAAACATG36000.11110541867299392No Hit
ATCGCAATGTAACTCG35830.11058075419592699No Hit
AGTGGTACTCAACGCC35790.1104573037307348No Hit
AACAGCTACTCGAGAG35350.10909934861362042No Hit
AGAACAATGAGTCGGA35110.10835864582246713No Hit
TTCACGCTGTGGGCAA34880.10764880564761187No Hit
GTCCTGACTTTACGAC34660.1069698280890547No Hit
TATCTCCCTGGAATTA34640.1069081028564586No Hit
TTCTATGACCAACGCC34620.10684637762386248No Hit
TGGTCCTTGTTTCTGT34560.10666120192607416No Hit
CATGTCAGAACAGGTG34440.10629085053049751No Hit
AGAGAATTGCCCGCAA33840.10443909355261427No Hit
TCCGGGATGCGAGTTT33770.10422305523852791No Hit
CCTTCTTTGGATCATC33630.10379097861035515No Hit
GTGTACTGAATCACAC33630.10379097861035515No Hit
GCATGGGCTATGTGGG33530.10348235244737461No Hit
ACCTCCATGGAATTGA33450.10323545151699018No Hit
AGGTTACTGGTCCCGA33290.10274164965622132No Hit
TGAGATAGACTCATCC33060.10203180948136607No Hit
GTTGCTAGACGCTCGA32970.1017540459346836No Hit
AGAACATGAAAGCTAA32830.10132196930651084No Hit
AAAGGATACTGCTGGC32470.1002109151197809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)