FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3185770
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT343681.0787972766395564No Hit
TGCTGCTACCGTTCTC316580.9937314997630087No Hit
AAACGACCTCTTCAAG310440.9744582942271414No Hit
ATGGGTTACAATCGAA300720.9439476170596119No Hit
TATGCTTTGGACTGTT293280.9205937654005155No Hit
GCCTCTTGATCTGTGA284280.8923431383935438No Hit
AAAGCGGACTCGGGCT272160.8542989606908221No Hit
TCCAAAGTGCTCACGG260650.8181695477074616No Hit
GCAGGTCTGGCTGTTA252920.7939053980670293No Hit
TAGTGCATGAACCCGC248230.7791836824378408No Hit
GAAGTATGAACAGGTG237350.7450318133449684No Hit
TAACTCACTGGTGTCC235400.7389108441601245No Hit
TGTGGCTCTTACGATC233570.7331665500020403No Hit
TTCCCATCTCTTTGTT208740.6552262090483619No Hit
GAGCCTGACCGAGTTT205630.645464047938175No Hit
TCACCTGACTCAATGC201950.63391268045088No Hit
GGAACTACTACCAAGG200210.6284508925628655No Hit
TGAGAATGAGCAGTTT192610.6045948075347561No Hit
CTAGAGACTAATCTGC185510.5823082017848118No Hit
GAAGTAATGAGTCTGT185430.5820570851003054No Hit
ATGTCCAGAGTCGGTC185060.5808956704344632No Hit
TTCGTCCGACCTGTGG180400.5662681235619647No Hit
TCGTATACTGCTAATC179980.564949760968306No Hit
TCACACAGATTGGGAG179720.56413363174366No Hit
TGCGGTGACCTTAGTG177350.5566942999651576No Hit
ATTCCTAACTGCTACA174260.5469949180260972No Hit
TCCGCGAGAGAACAGC171530.5384255611673159No Hit
AGGTCGAGAAGGGAGG168980.5304212168486739No Hit
ACTGATTCTCGGCTCA166980.5241432997360136No Hit
TGGACCTCTAGGGAGG166320.5220715870888357No Hit
TGGCCTGGACCCGAGT164540.5164842408585679No Hit
CCATGATGATTCGGGC157500.49438597262200346No Hit
GCAATCATGGAACGTG151180.4745477545459968No Hit
TTCGATGGAAATCCTG145240.4559023407213955No Hit
ACGAGGATGGAGCTGA142750.44808633391613334No Hit
AAGGTGCGAACAGATA137160.43053955558624757No Hit
TCCCAGACTGTCTTGA136930.42981759511829165No Hit
TTTCGTAACAGTACGG135120.42413608013133397No Hit
TGGTCCTTGGAGAGGG132150.41481337321903333No Hit
TCTCTCTACTCTGGAT127930.40156696811132No Hit
GCGGTTCGAGTACGTT126360.3966388031778816No Hit
TCCTTCACTTGTCACA116540.36581423015471926No Hit
TCGCCGAGAAGGTAAT114800.36035244226670476No Hit
TTCGATGGACAGGTGA114120.3582179504484002No Hit
TGATATCCTCGATGTT110090.3455679474663896No Hit
AGCCCAGTGAAAGTAC109620.3440926369449144No Hit
AGCTGCTCTTGTCACA108580.34082812004633106No Hit
AACTCCCTGCTTCACC106810.3352721634016266No Hit
AACCCTCACGCGGTTC106110.3330748924121955No Hit
CTTTCGGTGCTTTGTT105720.33185069857522675No Hit
CCCTTGCCTCCTACGG103200.32394052301327464No Hit
ATGGCCGTGATACGCT100480.3154025557400566No Hit
TCGGCTTACCTCCTAC98790.31009771577985856No Hit
TGTTTGTTGCAAGTTC93750.29427736465595444No Hit
AAACACGCTGCAGTTT93490.2934612354313086No Hit
ACCCTTGTGGGCCATC93480.29342984584574533No Hit
CTAACTGGAACCTTGA90760.2848918785725272No Hit
TGGCCTTTGCGAAAGC90080.2827573867542227No Hit
GGGGGGGGGGGGGGGG89290.28027760949472186No Hit
TACAACGTGAGCAGGT88150.2766991967405054No Hit
AAATAGGACTCTATCT87120.27346606942748536No Hit
TCAAAGGCTCTTAGTG86660.27202214849157347No Hit
CAATGTGTGGTTGACT85910.26966792957432584No Hit
TGAACTGTGTGCTGGC81320.2552601098007703No Hit
CTTTCGGTGAGCCATT80940.2540673055493648No Hit
GAGGTTGGAGGAATAT75610.237336656444125No Hit
AGTGTTCGATTCGCCT75530.23708553975961855No Hit
CAGCCGATGTAATAGG74260.23309906239307923No Hit
ACAGCCGACAAACATG72700.22820228704520415No Hit
GGCTTGCGAATACGCT71740.22518888683112714No Hit
GATAAGAACATTGGTC71040.22299161584169602No Hit
GTGCTAATGGGAGTCA67410.2115971962822175No Hit
CGACGTTCTTGCTACA67170.21084384622869823No Hit
GTAAAGCCTGAAATCA66200.20779905642905797No Hit
TTGGGAGGAATCTGTG64380.20208615185653706No Hit
TGTACTGGAAATGCTC63160.1982566224178142No Hit
TAACGAGCTTACTTTC60520.18996977182910255No Hit
AGGGACCCTAATCCTG60520.18996977182910255No Hit
GGACATTTGGCCGCTA60140.18877696757769707No Hit
GGGTGAAACCGACTAT59880.18796083835305122No Hit
GGAACATCTTTACGAC59760.1875841633262916No Hit
TCTTGAACTCGTACAT58770.18447659435552474No Hit
TACGTGTGAAACGCAG57580.18074123367349182No Hit
CGGATGAGAACAGGCA57170.17945426066539644No Hit
TACCACCTGAAACATG57160.17942287107983312No Hit
GGACCACTGGCTAGAG56990.178889248125257No Hit
CATGATGTGCCTAGTA54150.1699746058252793No Hit
AGCTTGTGAACCGTGC53870.16909569742950684No Hit
ACTTATCTGTCAGGTT53290.16727510146683533No Hit
AACTAGCACTGCCACG52670.16532894716191063No Hit
ACGTGTTGATCTTTGT50780.15939631549044658No Hit
TCGGACCGAATGTCCA50290.15785822579784478No Hit
TGGTAGGGATGTGCTA48230.1513919711718046No Hit
AACTACGACATTGCCA48200.1512978024151147No Hit
GTAGATAGAGCTGAGA47440.1489121939123038No Hit
CTCGGATCTAGTACGG47410.14881802515561388No Hit
CGTCGTACTTGCGCCA47400.14878663557005056No Hit
GTCCGAGTGGTCTTGA47060.14771938966089831No Hit
AACGAACCTGACACCC46330.14542794991477728No Hit
GGTAGTTTGTCCAGCG46280.14527100198696077No Hit
TTTAGGGACTGGGCAA46220.14508266447358095No Hit
CCTTCAGTGCAACGCC46010.1444234831767516No Hit
AGTTTGCGAGCAGGAT45210.14191231633168747No Hit
TACCACCTGTACTTTC44620.14006033078345265No Hit
TGAGGAGTGGTCCCAG44500.13968365575669303No Hit
GCCATAATGACCGTTA43700.1371724889116289No Hit
CTTTAACTGTTCGCCT43540.13667025554261608No Hit
CAGGCCATGTCGTAGT40580.12737893821587873No Hit
AGAACGCGACACAGTT40250.12634308189228977No Hit
GAGAAGAGATGCTACA40110.12590362769440355No Hit
GTCCGTTACGGATGGT39500.12398886297504215No Hit
CTTACATTGGAAGGCT39480.12392608380391552No Hit
GGGATGTGATGCATGT38970.12232521494018715No Hit
TGCAATGACTCCGTAA38870.12201131908455413No Hit
GAACCATACTTTGGGT38710.12150908571554128No Hit
GAAAGTCCTTATCTGG38340.12034767104969912No Hit
CCGTTACTGTTGGTTG38180.11984543768068631No Hit
CGATCGGTGCGAGGAT37570.1179306729613249No Hit
GAAGGACACCCAGCTG36930.11592173948527357No Hit
GCTGGACACATTGGTC36890.11579618114302037No Hit
AACTACGACGCTGTTA36540.11469754564830481No Hit
CCCACCTCTTGACCTA36470.11447781854936169No Hit
CAAAGACCTGCTTTGA36440.1143836497926718No Hit
AATTCTGCTTAGCGAG35690.11202943087542415No Hit
TCCTCATCTTTCTGGA35620.11180970377648103No Hit
GGCTTAAACTGCATCA35460.11130747040746822No Hit
TCCGTAAGATTACGCA35320.11086801620958198No Hit
TGTCTTCCTGAAATCA34440.10810573268001142No Hit
TCGTAGAACCGCACTG34300.10766627848212519No Hit
GCGTTTAGAAGTCTAC34210.10738377221205547No Hit
CCTGAGGACTGACTCA34190.10732099304092887No Hit
ACAAGACCTAAGCTAA33840.10622235754621331No Hit
ATGGCCGTGGGCCAAA33830.10619096796065002No Hit
ATAGCCAACATCTGTG33740.1059084616905803No Hit
TATGGGTACCCTTTGC32230.10116863427052172No Hit
GCGTTAATGGTACGTT32110.10079195924376211No Hit
AGGTGATCTGTTAGTC32110.10079195924376211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)