FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460927
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG235065495.51904627808952No Hit
GGCCAGGG345941.4057304422276646ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA284391.1556214385879793No Hit
GGACAGGC49450.20094053988598606No Hit
GGACACGG43300.17594995706902317No Hit
GGAAAGGG38560.15668892250765667No Hit
GGTCAGGG33320.13539613324572405No Hit
CGACAGGG32870.13356755401521458No Hit
GGACAGCG31690.1287726129218786No Hit
GGGCAGGG28140.1143471545478594No Hit
GCACAGGG26550.10788617460005924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)