FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18631416
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC19400.011.90644922
ATCTCCG22700.010.360455510
CACGAGA22900.010.26999921
CCCACGA25450.09.57093919
CTTATAC27450.09.4900571
TCTCCGA24650.09.42723311
TATACAC41700.09.06679253
TATACTG22250.08.999835
TAGACTG26500.08.6661515
TAGACAG34400.08.6298995
TTATACA36400.08.617352
CCACGAG28600.08.61466820
TCCGAGC30750.08.01236313
TACACTG33450.07.99586635
GTACCGT6850.07.9726656
GTATTAG21200.07.92763471
CTAGTAC13550.07.75082543
CTAGGAC29700.07.6852343
TACACAT45650.07.6688025
CTAGACT25050.07.490694
Produced by FastQC (version 0.11.5)