FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3409075
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG333074397.70225061050284No Hit
TAATGGTG84030.24648915028270135No Hit
TATTGGTA78450.23012107389834488No Hit
AATTGGTG68600.20122760572882673No Hit
TATTGGGG60880.178582166716778No Hit
TATTGGTC54200.15898740860790683No Hit
TATTGCTG51810.15197670922464304No Hit
TATTGGAG51510.1510967051179572No Hit
TATTCGTG47530.1394219839692585No Hit
TATAGGTG44310.12997660655749727RNA PCR Primer, Index 39 (100% over 8bp)
TATTGGTT43340.12713125994587973No Hit
TTTTGGTG34770.10199247596488782RNA PCR Primer, Index 28 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)