FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418019
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT334471197.85524890294641No Hit
CTAGCTGA186570.5458424894653892No Hit
CTAGCTAT54560.15962462467294652RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCAGT53800.15740111450521485No Hit
CAAGCTGT52400.15330517472255126No Hit
CTAGATGT43070.12600866174237182No Hit
CTAGCTGG35380.10351024965045542No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)