FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004949751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436645
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC28480.6522461038143114No Hit
CTCCTAGTGAACGTAA22820.522621351441102No Hit
CGACCAGACGCGCTGT20020.4584960322458748No Hit
TAGCTTTACCCTATCC19610.4491062533637165No Hit
CCAGGACTGTAACTCG15100.3458186856599755No Hit
ATCTGTACTGGGACTA14550.33322264081805586No Hit
GTTCTCGACCGTAGAG14270.3268101088985331No Hit
CTTCCTTTGGCTCAGC14250.32635207090428153No Hit
CGATCAAACCCGGCTA12670.2901670693584033No Hit
TGCTGCTACTTTCTTG11360.26016558073492196No Hit
ACAGCCGACTTGCGTA10990.25169187784126695No Hit
GGGGGGGGGGGGGGGG10450.2393248519964731No Hit
GGCAACAGAATAGCAA10080.2308511491028181No Hit
CGCGTTGGAGCGGTTC9420.21573589529251452No Hit
AACTTACCTCACCTAA9330.21367472431838222No Hit
ATCGAAAGATTTCCGC9190.21046845835862082No Hit
GTAACTGTGTGAAAGT9180.21023943936149503No Hit
CCCTGAACTAGCTTTG9110.20863630638161437No Hit
CAATTCGGATAACGGG8220.1882536156374171No Hit
AGTGGAGTGAGTTGAA8140.18642146366041062No Hit
GGGTAGAACCAGTCTA7820.17909285575238465No Hit
GTGAAGCGATCCCAGG7570.17336738082423936No Hit
AAGAACCCTTAACCCA7460.17084817185585544No Hit
TTTAGTCTGAGCAATA7090.16237446896220042No Hit
AGTCCTGCTCGAACGA6830.15641997503692934No Hit
CACATCCTGAGCCTAT6620.15161057609728726No Hit
TGGAACACTCCTAGTA6600.15115253810303564No Hit
TGATAGAGAGACTGTT6530.14954940512315495No Hit
TTCCTAAACTAATGCC6520.14932038612602916No Hit
TCCGGGAACGTTCTCC6460.1479462721432743No Hit
TGCAGATACTGCCACG6450.14771725314614848No Hit
CGCGATCGATATCTGG6370.145885101169142No Hit
CTTTAACTGTCCGTAA6360.14565608217201617No Hit
TGCAGTATGACACCGC6260.14336589220075804No Hit
GCAACTTGAAGGTGCA6230.14267883520938063No Hit
CATAGGTACAAGGTGC6230.14267883520938063No Hit
GAGTGTTACACCTGCG6100.13970158824674506No Hit
ATTTCTGACGGGTAGT6040.1383274742639902No Hit
TCAAAGCACTCTGCAA6030.1380984552668644No Hit
CTATTGGGACTTGTGA6000.13741139827548696No Hit
AGACCATTGAATCCAC5960.13649532228698372No Hit
TCTCAGAGATGCATGT5940.1360372842927321No Hit
AGAGTACCTTAATCGC5890.13489218930710303No Hit
TGAACTGTGAACGTAA5880.1346631703099772No Hit
TACGTTTACTGCACCG5860.1342051323157256No Hit
CTTAGATCTTGTCACA5840.13374709432147397No Hit
GGTCGCATGGTACTAC5800.13283101833297073No Hit
TGGCAATTGAGTGGTG5780.1323729803387191No Hit
ATGTACGTGTACTTTC5690.1303118093645868No Hit
TCTATGGGATGCTCAA5660.12962475237320936No Hit
TGAGGGATGTACGTTG5570.12756358139907706No Hit
AGGTGTGGATCATGTG5560.12733456240195123No Hit
GGTCTAAACTCCTACC5560.12733456240195123No Hit
TCCATTTGAAATCTGC5490.12573142942207055No Hit
TTGCGGTCTTTTGGGT5380.12321222045368664No Hit
GGCTGACTGAAACTGA5340.12229614446518339No Hit
CGAGTCTCTGCTACGC5340.12229614446518339No Hit
TACTGTTTGCTTAAGC5300.12138006847668013No Hit
TTCTTAGACGTCCCGA5230.11977693549679946No Hit
CACATTACTGATGGAC5170.1184028215140446No Hit
CACCAGGTGCGCCACA5150.11794478351979296No Hit
CTCAATTTGGAATCTC5140.11771576452266716No Hit
GTAATCTCTACACAAA5140.11771576452266716No Hit
GGGTGTGCTCGCAAAG5120.11725772652841553No Hit
GCATGATACTTGCGTA5110.11702870753128973No Hit
TCGGACCGATAGGCGA5110.11702870753128973No Hit
AGACTACTGGCCCTAG5090.1165706695370381No Hit
AGCCAATTGTTATGGC5070.11611263154278648No Hit
GGGATGTGACTGGAAG5020.11496753655715741No Hit
CATTCTATGCCTAGCG4950.11336440357727674No Hit
TGCGCAGACATCAGGG4900.11221930859164767No Hit
ATGTCCAGACCGCTAG4890.11199028959452187No Hit
CTACAACGAATCTGTG4830.11061617561176701No Hit
CTAGCTCACGAATAGT4820.11038715661464119No Hit
TGTAAGCACGCGCTGT4720.10809696664338306No Hit
GTCTAATCTAAGTTCC4720.10809696664338306No Hit
ATAGCCAACCGTTCTC4700.10763892864913145No Hit
CTTTCGTCTCGAACGA4700.10763892864913145No Hit
AGGCGATGACCTGCGA4660.1067228526606282No Hit
TCCGGGTGAACACCTA4640.10626481466637658No Hit
TCATCATTGGACTCGC4620.10580677667212496No Hit
ACCAAGTTGAAGCTAA4610.10557775767499915No Hit
CTAACAGTGCATGATC4610.10557775767499915No Hit
AGCTTGTGATTGCGTA4600.10534873867787332No Hit
ACCTCAGCTAATCCGT4600.10534873867787332No Hit
TGCTAGTGAAGGCGTA4550.10420364369224427No Hit
CATAGTCGAAGATTCC4550.10420364369224427No Hit
GTATTCACTGCTCCTT4490.10282952970948941No Hit
TCCCAGACTAGTACGG4420.10122639672960872No Hit
AGTTTGCGAAACGAAT4380.10031032074110546No Hit
ACGAGGATGAACTAGA4370.10008130174397967No Hit
GGGCCTTGACTATCTT4370.10008130174397967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)