FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384498
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT517474096.10440936183838No Hit
CTTTCCTA980561.8210796995374499No Hit
CTTTCCAT145480.2701830328472589No Hit
CTTTACTT133900.2486768497267526No Hit
CTTTCATT108800.20206154779888488No Hit
CTATCCTT93290.17325663413748135No Hit
CATTCCTT90540.16814937994219703TruSeq Adapter, Index 27 (100% over 8bp)
ATTTCCTT87490.16248497074379079No Hit
CTTACCTT73290.13611296726268632No Hit
CGTTCCTT65050.12080977651027078No Hit
CTTTCCGT56600.10511657725566989No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)