FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16360449
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184670.11287587522811873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA34200.09.7167551
TATACCG3201.2732926E-119.6236685
GTCCTAG22950.08.7367981
TAGACTG20950.08.4188815
GTCTAGA19050.07.949281
GTATAAG28800.07.92510653
TGTATAA33900.07.89272642
TAGGTCG4553.6379788E-117.6912425
TACACTG27850.07.6901385
TGTACCG4751.2732926E-117.66209655
TACACCG7150.07.63530545
GTATTAG16250.07.50697371
TAGACAG30100.07.440835
TCTATAC14350.07.41603333
CTAGGAC22200.07.37977173
TAGGACG6500.07.3225334
GTGTTAG21500.07.30429941
TATACTG18800.07.2968615
GTCTTAG19650.07.27841141
GTACTAG9650.07.2651021
Produced by FastQC (version 0.11.5)