FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773925
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG271957098.04050217651883No Hit
TATTGGTA63550.22909775859116596No Hit
TAATGGTG57220.20627810773542907No Hit
TATTGGGG53490.19283145723117964No Hit
AATTGGTG43840.15804320592662022No Hit
TATTGGAG35490.12794145479780455No Hit
TTTTGGTG33350.1202267545085033RNA PCR Primer, Index 28 (100% over 8bp)
TATAGGTG30030.10825815405968078RNA PCR Primer, Index 39 (100% over 8bp)
TATTGGTC29360.10584280396910516No Hit
TATTGGTT28630.10321115387041827No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)