FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774424
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC368868197.72831563173612Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA187020.49549282221605206No Hit
ATATGATC96650.2560655612617978No Hit
ATCAGATC94330.2499189280271639Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGCTC85850.227451923790226No Hit
ATCTGAAC54450.14426042225250793No Hit
AACTGATC52230.13837873010557372No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)