FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541427
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC249116098.02209546054245Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA110220.43369335416677324No Hit
ATATGATC60400.23766175459692526No Hit
ATCTGCTC58890.23172021073200214No Hit
ATCAGATC48460.1906802752941556Illumina PCR Primer Index 9 (100% over 8bp)
AACTGATC34420.1354357217421551No Hit
ATCTGAAC34300.13496354607077046No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)