FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541427
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT435791.714745298605862No Hit
GAAGCAGTGGAACCTT166680.6558520075532368No Hit
GTTCTTCCTAACGTAA131540.517583231782774No Hit
GATACGTACCGCTCGA119440.46997218491815823No Hit
CGCTTAAGATAATAGG102650.40390693889692686No Hit
TGTCCCATGAGACAAT99970.3933616822360036No Hit
CATGGAGGATCGACAT99370.39100080387908054No Hit
AAAGCATGATCTTTGT94620.3723105168867727No Hit
TGAGCGCACAGGACAC94040.3700283344750803No Hit
TGTCGAAACTTCGCAA85330.33575625032708No Hit
TAATTGCCTGTCTGGC85010.3344971152033877No Hit
GGAAGGACTAGGTAAT77140.3035302607550797No Hit
GGGGGGGGGGGGGGGG67170.26430033205754094No Hit
AATGATGGAAAGCTAA66720.2625296732898486No Hit
CATGTCAGAGGTTTGT65520.2578079165760024No Hit
TCGACCTTGTTTCGCC64130.2523385483824639No Hit
GTGAGATCTCGATTAC55720.2192469034129251No Hit
ATGGGTACTTGGCTGC51250.201658359653848No Hit
AAACGACCTAGGGAGG50390.19827443400892492No Hit
AGCCTACCTTAACTCG48150.18946048814307867No Hit
CAGCTCACTGTTTACG47800.1880833091015402No Hit
TGTTCCTCTTTATGGC47700.18768982937538636No Hit
GCTATGATGACCTGCG47330.18623395438861712No Hit
TTGCGATGACATTCAG46860.184384599675694No Hit
CTGCCTTGACCATAAC42720.16809453901292462No Hit
CCTGTTGACCCTAGTA42700.16801584306769385No Hit
CAATGTGACCAACGCC42560.16746497145107847No Hit
CATGCAAACGAAGGCT41450.16309734649077073No Hit
TAACCAGACATCACAC40870.16081516407907842No Hit
CACAGCTCTGGACTGA40490.15931994111969378No Hit
CATGATGTGTCATGTG40050.15758863032461684No Hit
ATGGGTACTTTCGGGC39890.1569590627627707No Hit
AGCTTGTGACTCACTC39760.15644753911877066No Hit
ATGCCGCGATGCCACG39420.15510970804984758No Hit
CTATTCAACCGAACGA39320.15471622832369372No Hit
TGGGCCATGCGTTCTC38790.15263078577507833No Hit
TTGTTTGTGATACGCT38020.1496009918836937No Hit
AGAACGCGAGGAATAT37610.1479877250064629No Hit
ATTAGCGTGGTCGGTC36990.14554815070430904No Hit
CATTAGTCTATTCTGG36810.1448398871972321No Hit
GGGCTGACTAATACAG35890.14121987371661668No Hit
CACTATACTACGCCAC35600.14007878251077052No Hit
CCGCTATTGTTCGCAA35570.13996073859292435No Hit
GGGTCGTACCGCTCGA35380.13921312711323205No Hit
AACCAACACCGGAATG34780.13685224875630897No Hit
GGTAGCCTGTGCCACG34470.135632461605232No Hit
AACGTCCCTTACAGAA34060.13401919472800122No Hit
CCCAATGCTGGAATGC33870.1332715832483089No Hit
AGCTCGGACCTTAAGC33720.13268136365907815No Hit
TTGTAACCTCATCCTC33510.13185505623415505No Hit
TTGACGTGACCTCAAA33430.13154027245323197No Hit
ATAGGAAACAGACTTC33370.13130418461753968No Hit
GCAGATACTCGGCTCA33240.13079266097353967No Hit
GGCTTGCGAGTACGTT33210.13067461705569353No Hit
TGGAGTTACAAGTCTC32090.1262676441227704No Hit
CTGCCATTGCACAGTT31970.12579546845138578No Hit
ATAGCCAACATGGCCC31910.12555938061569347No Hit
CTGATTACTGCTTTGA31840.12528394480738578No Hit
TCAGGCTCTTAAAGCA31680.12465437724553963No Hit
CCTACGGGATGGAACG31420.12363132995753962No Hit
CGCTTACTGGCCACGT31190.12272632658738576No Hit
TGTTTGTTGCGCTCGA30780.12111305971015496No Hit
TCCAAAGTGAAGGCCA30730.12091631984707804No Hit
GATACCATGTCTGGAT30600.12040479620307803No Hit
CGCTGAGTGTTAGCTG30380.11953914080553958No Hit
CGATCAAACCGATCGC30220.1189095732436934No Hit
CGCTTCCCTGCGCTGT29980.11796522190092418No Hit
GCGTCGATGCTTGAAC29960.11788652595569339No Hit
TGGGTGCACTTCTGAG29850.11745369825692416No Hit
GTAAGAAACGACAAAT29790.11721761042123185No Hit
CTCGGATCTTTCTGAG29750.11706021853077032No Hit
TTCTAACACTACGTTG29640.11662739083200108No Hit
ATCTTACTGCGCACTG29130.11462064422861644No Hit
CATGTTACTAACGTGG28890.1136762928858472No Hit
CTTAGGCCTTATGACC28490.11210237398123181No Hit
AGCCGGTACGCGCAAA28480.11206302600861642No Hit
CGAGTCTCTTACTTTC28460.11198433006338565No Hit
ACCTGCTTGCCAAACT28370.11163019830984719No Hit
CCTTCAGTGTAGCTTG28290.1113154145289241No Hit
AGTGGTACTCAACGCC28200.11096128277538564No Hit
TACGCCTTGGGTTGAG27670.10887584022677024No Hit
CCTCCCTTGTTTCGCC27540.10836431658277022No Hit
GCGACGTTGCTTAGTG27410.10785279293877023No Hit
AGAACAATGAGTCGGA27040.10639691795200099No Hit
GAAAGTAACCCTGCGA27010.10627887403415483No Hit
ATCGCAATGTAACTCG26990.10620017808892405No Hit
GTCCTGACTTTACGAC26970.10612148214369328No Hit
AACCACTGAAAACATG26870.10572800241753944No Hit
ATTGAGAGATGATCAC26730.10517713080092404No Hit
TACAACCGATTCCATT26550.10446886729384712No Hit
TGAGATAGACTCATCC26530.10439017134861635No Hit
AGAACATGAAAGCTAA26510.10431147540338556No Hit
GCGACAAGAAAGGCTG26470.10415408351292403No Hit
AACAGCTACTCGAGAG26410.10391799567723173No Hit
GTGTACTGAATCACAC26330.10360321189630864No Hit
CATGTCAGAACAGGTG26170.1029736443344625No Hit
TGGTCCTTGTTTCTGT26060.10254081663569325No Hit
GGTCAGAGAACCAGTT25900.10191124907384709No Hit
TTCTATGACCAACGCC25880.10183255312861633No Hit
TTCACGCTGTGGGCAA25840.10167516123815477No Hit
TATCTCCCTGGAATTA25800.10151776934769324No Hit
TCCGGGATGCGAGTTT25630.1008488538132317No Hit
ACCTCCATGGAATTGA25540.10049472205969325No Hit
GTTGCTAGACGCTCGA25470.10021928625138553No Hit
AGGTTACTGGTCCCGA25450.10014059030615477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)