FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497962
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT271791.0880469758947493No Hit
TGCTGCTACCGTTCTC255121.0213125740103333No Hit
AAACGACCTCTTCAAG245820.9840822238288653No Hit
ATGGGTTACAATCGAA239140.9573404239135743No Hit
TATGCTTTGGACTGTT238840.9561394448754624No Hit
GCCTCTTGATCTGTGA227450.9105422740618152No Hit
AAAGCGGACTCGGGCT219460.8785561990134357No Hit
TCCAAAGTGCTCACGG206820.8279549488743223No Hit
TAGTGCATGAACCCGC195860.7840791813486354No Hit
TGTGGCTCTTACGATC188780.7557360760491953No Hit
GCAGGTCTGGCTGTTA186420.7462883742827153No Hit
TAACTCACTGGTGTCC184120.7370808683238577No Hit
GAAGTATGAACAGGTG176490.7065359681212124No Hit
TTCCCATCTCTTTGTT168560.6747900888804553No Hit
GAGCCTGACCGAGTTT161780.647647962619127No Hit
TCACCTGACTCAATGC158380.6340368668538593No Hit
TGAGAATGAGCAGTTT156450.6263105683753396No Hit
GGAACTACTACCAAGG154290.6176635193009341No Hit
CTAGAGACTAATCTGC149290.5976472019990696No Hit
ATGTCCAGAGTCGGTC146850.5878792391557598No Hit
TCGTATACTGCTAATC146370.5859576726947807No Hit
TTCGTCCGACCTGTGG144470.5783514721200722No Hit
ATTCCTAACTGCTACA140640.563018973066844No Hit
TCACACAGATTGGGAG139280.5575745347607369No Hit
TCCGCGAGAGAACAGC137290.5496080404745949No Hit
ACTGATTCTCGGCTCA135080.5407608282271709No Hit
AGGTCGAGAAGGGAGG133950.5362371405169494No Hit
TGGCCTGGACCCGAGT130900.524027186962812No Hit
TGGACCTCTAGGGAGG129480.5183425528490826No Hit
CCATGATGATTCGGGC129320.517702030695423No Hit
GCAATCATGGAACGTG125750.5034103801418917No Hit
TTCGATGGAAATCCTG113600.454770729098361No Hit
ACGAGGATGGAGCTGA110760.443401460870902No Hit
AAGGTGCGAACAGATA109080.43667597825747545No Hit
TTTCGTAACAGTACGG107020.4284292555291073No Hit
TGCGGTGACCTTAGTG106980.4282691249906924No Hit
TCCCAGACTGTCTTGA105530.4224643929731517No Hit
TGGTCCTTGGAGAGGG102510.41037453732282553No Hit
TCTCTCTACTCTGGAT102370.4098140804383733No Hit
TTCGATGGACAGGTGA91360.3657381497396678No Hit
TGATATCCTCGATGTT90320.36157475574087994No Hit
AGCTGCTCTTGTCACA89860.3597332545491084No Hit
TCCTTCACTTGTCACA89740.3592528629338637No Hit
TCGCCGAGAAGGTAAT88730.35520956683888705No Hit
AGCCCAGTGAAAGTAC88060.35252738032043723No Hit
CTTTCGGTGCTTTGTT88020.35236724978202233No Hit
AACTCCCTGCTTCACC87560.35052574859025076No Hit
GAAGTAATGAGTCTGT86220.3451613755533511No Hit
AACCCTCACGCGGTTC83800.3354734779792487No Hit
CCCTTGCCTCCTACGG83750.33527331480623No Hit
ATGGCCGTGATACGCT82460.330109104942349No Hit
TCGGCTTACCTCCTAC77720.31113363614018147No Hit
AAACACGCTGCAGTTT76260.305288871488037No Hit
TGTTTGTTGCAAGTTC76190.30500864304581093No Hit
GGGGGGGGGGGGGGGG75060.30048495533558955No Hit
ACCCTTGTGGGCCATC73420.29391960326057803No Hit
TGGCCTTTGCGAAAGC72800.29143757991514685No Hit
CTAACTGGAACCTTGA71550.2864335005896807No Hit
AAATAGGACTCTATCT70040.2803885727645176No Hit
CAATGTGTGGTTGACT69570.2785070389381423No Hit
TACAACGTGAGCAGGT69570.2785070389381423No Hit
ACAGCCGACAAACATG67640.2707807404596227No Hit
TCAAAGGCTCTTAGTG67110.26865901082562504No Hit
GGACATTTGGCCGCTA66520.26629708538400504No Hit
CTTTCGGTGAGCCATT65780.2633346704233291No Hit
TGAACTGTGTGCTGGC63990.2561688288292616No Hit
CAGCCGATGTAATAGG62450.25000380310028736No Hit
AGTGTTCGATTCGCCT60740.2431582225830497No Hit
GGCTTGCGAATACGCT56860.22762556035680284No Hit
GATAAGAACATTGGTC53190.21293358345723434No Hit
GTGCTAATGGGAGTCA53140.21273342028421569No Hit
GTAAAGCCTGAAATCA51770.20724894934350482No Hit
TTGGGAGGAATCTGTG51150.20476692599807364No Hit
TGTACTGGAAATGCTC50510.20220483738343495No Hit
CGACGTTCTTGCTACA50130.20068359726849327No Hit
GGACCACTGGCTAGAG48780.19527919159698987No Hit
GGAACATCTTTACGAC48070.1924368745401251No Hit
AGGGACCCTAATCCTG47870.19163622184805054No Hit
TAACGAGCTTACTTTC47020.18823344790673358No Hit
TACCACCTGAAACATG46670.18683230569560305No Hit
GGGTGAAACCGACTAT46520.18623181617654713No Hit
TCTTGAACTCGTACAT45250.18114767158187356No Hit
AGCTTGTGAACCGTGC45200.1809475084088549No Hit
CGGATGAGAACAGGCA45050.18034701888979895No Hit
TACGTGTGAAACGCAG44780.17926613775549827No Hit
CATGATGTGCCTAGTA43060.1723805246036569No Hit
ACTTATCTGTCAGGTT42870.17161990454618606No Hit
AACTAGCACTGCCACG42320.16941810964298096No Hit
TCGGACCGAATGTCCA38710.1549663285510348No Hit
AACTACGACATTGCCA38600.15452596957039377No Hit
ACGTGTTGATCTTTGT38310.15336502316688566No Hit
GTCCGAGTGGTCTTGA38030.15224410939798122No Hit
TGGTAGGGATGTGCTA37710.1509630650906619No Hit
CCTTCAGTGCAACGCC37440.14988218395636121No Hit
CGTCGTACTTGCGCCA37440.14988218395636121No Hit
GTAGATAGAGCTGAGA37420.14980211868715376No Hit
AACGAACCTGACACCC37210.14896143336047546No Hit
CTCGGATCTAGTACGG36970.14800065012998595No Hit
AGTTTGCGAGCAGGAT36790.14728006270711885No Hit
TACCACCTGTACTTTC36190.14487810463089512No Hit
GGTAGTTTGTCCAGCG36110.14455784355406526No Hit
GCCATAATGACCGTTA35830.14343692978516087No Hit
TTTAGGGACTGGGCAA35730.14303660343912358No Hit
TGAGGAGTGGTCCCAG35200.14091487380512593No Hit
CTTTAACTGTTCGCCT33810.13535033759520762No Hit
GGGATGTGATGCATGT31190.12486178732903062No Hit
CTTACATTGGAAGGCT31180.1248217546944269No Hit
GAACCATACTTTGGGT31150.1247016567906157No Hit
AGAACGCGACACAGTT31090.12446146098299334No Hit
GTCCGTTACGGATGGT31020.12418123254076723No Hit
CAGGCCATGTCGTAGT30900.1237008409255225No Hit
AATTCTGCTTAGCGAG30610.12253989452201434No Hit
CGATCGGTGCGAGGAT30370.12157911129152485No Hit
CCGTTACTGTTGGTTG30230.12101865440707264No Hit
GAAAGTCCTTATCTGG30140.12065836069563908No Hit
GAGAAGAGATGCTACA30100.12049823015722416No Hit
CAAAGACCTGCTTTGA30090.12045819752262044No Hit
TGCAATGACTCCGTAA30010.12013793644579061No Hit
GAGGTTGGAGGAATAT29720.11897699004228246No Hit
AACTACGACGCTGTTA29130.11661506460066247No Hit
TCCTCATCTTTCTGGA28590.1144533023320611No Hit
GCTGGACACATTGGTC28120.11257176850568583No Hit
CCCACCTCTTGACCTA27690.1108503652177255No Hit
TGTCTTCCTGAAATCA27490.11004971252565092No Hit
CCTGAGGACTGACTCA27260.10912896192976514No Hit
GGCTTAAACTGCATCA27200.10888876612214277No Hit
ATGGCCGTGGGCCAAA27010.10812814606467193No Hit
ACAAGACCTAAGCTAA26860.10752765654561598No Hit
TCGTAGAACCGCACTG26490.10604644906527802No Hit
ATAGCCAACATCTGTG26320.10536589427701464No Hit
TCCGTAAGATTACGCA26320.10536589427701464No Hit
TATGGGTACCCTTTGC25850.10348436045063937No Hit
AGGTGATCTGTTAGTC25230.10100233710520817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)