FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053094
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG198318496.59489531409668No Hit
GGACAGGA238151.1599566313086493No Hit
GGCCAGGG181110.8821320407151353ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGGCAGGG29820.1452442021651225No Hit
GGACAGGC25090.12220580255945417No Hit
GGAAAGGG22320.1087139702322446No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)