FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774424
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT646581.7130560848489729No Hit
GAAGCAGTGGAACCTT235300.6234063793574861No Hit
GTTCTTCCTAACGTAA192250.5093492411027484No Hit
GATACGTACCGCTCGA153490.4066580755103295No Hit
CGCTTAAGATAATAGG153440.4065256049664796No Hit
CATGGAGGATCGACAT153260.4060487110086201No Hit
TGTCCCATGAGACAAT148810.39425883260598177No Hit
AAAGCATGATCTTTGT142790.3783093791264574No Hit
TGAGCGCACAGGACAC135760.3596840206611658No Hit
TGTCGAAACTTCGCAA125980.33377278228413126No Hit
TAATTGCCTGTCTGGC125320.332024171105313No Hit
GGAAGGACTAGGTAAT111450.2952768422413592No Hit
GGGGGGGGGGGGGGGG109980.29138220825217304No Hit
AATGATGGAAAGCTAA101620.2692331333204749No Hit
CATGTCAGAGGTTTGT99960.2648351112646592No Hit
TCGACCTTGTTTCGCC93240.24703107017123674No Hit
GTGAGATCTCGATTAC78390.20768731864782547No Hit
ATGGGTACTTGGCTGC76460.2025739556552205No Hit
AAACGACCTAGGGAGG74380.19706318103106593No Hit
AGCCTACCTTAACTCG71830.19030718329472257No Hit
TGTTCCTCTTTATGGC70320.18630657287045654No Hit
TTGCGATGACATTCAG69820.18498186743195782No Hit
CAGCTCACTGTTTACG69590.18437250293024843No Hit
GCTATGATGACCTGCG66050.17499358842567767No Hit
CCTGTTGACCCTAGTA64300.17035711939093223No Hit
CTGCCTTGACCATAAC63870.16921787271382335No Hit
CAATGTGACCAACGCC63420.16802563781917454No Hit
TAACCAGACATCACAC62040.16436945080891813No Hit
AGCTTGTGACTCACTC61470.16285928660902962No Hit
CATGCAAACGAAGGCT60230.15957401712155286No Hit
ATGCCGCGATGCCACG60200.15949453479524292No Hit
CATGATGTGTCATGTG60010.15899114672861342No Hit
CACAGCTCTGGACTGA59920.15875269974968365No Hit
ATGGGTACTTTCGGGC59120.15663317104808575No Hit
CTATTCAACCGAACGA58670.1554409361534369No Hit
TGGGCCATGCGTTCTC57600.1526060665150497No Hit
AGAACGCGAGGAATAT56930.15083096122746148No Hit
TTGTTTGTGATACGCT56550.14982418509420245No Hit
ATTAGCGTGGTCGGTC56390.14940027935388286No Hit
CATTAGTCTATTCTGG54690.1448962808629873No Hit
CCGCTATTGTTCGCAA54290.14383651651218834No Hit
CACTATACTACGCCAC53680.14222037587721995No Hit
CCCAATGCTGGAATGC52160.1381932713441839No Hit
GGGTCGTACCGCTCGA51370.136100236751356No Hit
AACCAACACCGGAATG51300.13591477798996615No Hit
AACGTCCCTTACAGAA50340.13337134354804867No Hit
AGCTCGGACCTTAAGC49980.1324175556323296No Hit
TTGACGTGACCTCAAA49840.13204663810954995No Hit
GGGCTGACTAATACAG49770.13186117934816016No Hit
GGTAGCCTGTGCCACG49600.13141077949907057No Hit
ATAGGAAACAGACTTC49580.13135779128153063No Hit
TTGTAACCTCATCCTC49410.1309073914324411No Hit
CTGCCATTGCACAGTT48960.12971515653779225No Hit
CTGATTACTGCTTTGA48030.12725120442218468No Hit
TGGAGTTACAAGTCTC47840.12674781635555518No Hit
CCTACGGGATGGAACG47380.1255290873521364No Hit
ATAGCCAACATGGCCC47310.12534362859074655No Hit
GGCTTGCGAGTACGTT46810.12401892315224787No Hit
CGCTGAGTGTTAGCTG46000.12187290034187998No Hit
CGCTTACTGGCCACGT45930.12168744158049015No Hit
CGATCAAACCGATCGC45550.12068066544723116No Hit
TGTTTGTTGCGCTCGA45420.12033624203322149No Hit
TCAGGCTCTTAAAGCA44810.11872010139825308No Hit
TCCAAAGTGAAGGCCA44340.1174748782860643No Hit
GCAGATACTCGGCTCA43650.11564678478093611No Hit
CGCTTCCCTGCGCTGT43620.11556730245462618No Hit
CTCGGATCTTTCTGAG43480.11519638493184656No Hit
ATCTTACTGCGCACTG43370.11490494973537685No Hit
TTCTAACACTACGTTG43260.11461351453890714No Hit
AGCCGGTACGCGCAAA43080.11413662058104761No Hit
CATGTTACTAACGTGG43060.11408363236350766No Hit
TGGGTGCACTTCTGAG43000.11392466771088781No Hit
CGAGTCTCTTACTTTC42850.1135272560793382No Hit
GTAAGAAACGACAAAT42850.1135272560793382No Hit
TACGCCTTGGGTTGAG41640.11032146891817136No Hit
AGTGGTACTCAACGCC41600.11021549248309147No Hit
GCGTCGATGCTTGAAC41380.10963262209015202No Hit
ACCTGCTTGCCAAACT41330.10950015154630216No Hit
CTTAGGCCTTATGACC41310.10944716332876221No Hit
CCTCCCTTGTTTCGCC41010.10865234006566302No Hit
TGAGATAGACTCATCC40720.10788401091133375No Hit
GATACCATGTCTGGAT40540.10740711695347421No Hit
CCTTCAGTGTAGCTTG40290.10674476423422488No Hit
AGAACAATGAGTCGGA40200.10650631725529511No Hit
GCGACGTTGCTTAGTG40050.1061089056237455No Hit
ATCGCAATGTAACTCG40020.10602942329743557No Hit
AACAGCTACTCGAGAG39560.10481069429401678No Hit
ATTGAGAGATGATCAC39540.10475770607647683No Hit
TACAACCGATTCCATT39410.10441328266246719No Hit
GTCCTGACTTTACGAC39320.10417483568353741No Hit
TATCTCCCTGGAATTA38940.10316805955027838No Hit
TGGTCCTTGTTTCTGT38910.10308857722396848No Hit
TTCACGCTGTGGGCAA38610.10229375396086926No Hit
AACCACTGAAAACATG38570.10218777752578938No Hit
ACCTCCATGGAATTGA38290.10144594248023009No Hit
TCCGGGATGCGAGTTT38190.10118100139253035No Hit
CATGTCAGAACAGGTG38080.10088956619606064No Hit
TCGACCTTGGGACCTC38010.10070410743467083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)