FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926518
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT282111896.39845030852364No Hit
CTTTCCTA530801.813759559995872No Hit
CTTTCCAT72970.2493406840484152No Hit
CTTTACTT55550.18981602026708874No Hit
CTTTCATT53150.18161514810433424No Hit
CATTCCTT39730.13575860459426528TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT37500.1281386275430392No Hit
ATTTCCTT32320.11043841179176073No Hit
CTTACCTT29430.10056319489577717No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)