FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712819
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT401791.0821696398343146No Hit
TGCTGCTACCGTTCTC380161.0239120194116653No Hit
AAACGACCTCTTCAAG367850.9907566191618821No Hit
ATGGGTTACAATCGAA359730.9688864445048357No Hit
TATGCTTTGGACTGTT354900.9558774613036617No Hit
GCAGGTCTGGCTGTTA335810.9044610038894974No Hit
AAAGCGGACTCGGGCT328730.8853919353461615No Hit
TCCAAAGTGCTCACGG314840.8479810085005489No Hit
GAAGTATGAACAGGTG295270.795271732880057No Hit
TAGTGCATGAACCCGC293890.7915548805368643No Hit
TGTGGCTCTTACGATC278750.750777239612273No Hit
TAACTCACTGGTGTCC275880.7430472640869377No Hit
TTCCCATCTCTTTGTT249790.6727772078304921No Hit
GCCTCTTGATCTGTGA244240.6578289973198262No Hit
TCACCTGACTCAATGC239320.6445776107049657No Hit
TGAGAATGAGCAGTTT234640.6319726331932691No Hit
GGAACTACTACCAAGG231280.622922905748974No Hit
GAAGTAATGAGTCTGT222080.5981438901276901No Hit
ATGTCCAGAGTCGGTC218620.5888248255570767No Hit
CTAGAGACTAATCTGC218440.5883400187297038No Hit
TGCGGTGACCTTAGTG215720.5810140488938459No Hit
TCGTATACTGCTAATC214900.5788054844580358No Hit
TCACACAGATTGGGAG214350.5773241302632851No Hit
TTCGTCCGACCTGTGG211580.5698634918642681No Hit
AGGTCGAGAAGGGAGG209260.5636148705336834No Hit
CCATGATGATTCGGGC209170.5633724671199969No Hit
TCCGCGAGAGAACAGC207360.5584974651336356No Hit
ATTCCTAACTGCTACA207150.5579318571683672No Hit
ACTGATTCTCGGCTCA201220.5419601655776918No Hit
TGGACCTCTAGGGAGG199070.5361694173618483No Hit
GCAATCATGGAACGTG192010.517154216243776No Hit
TGGCCTGGACCCGAGT186330.5018558674688963No Hit
TTCGATGGAAATCCTG173640.46767698613910347No Hit
ACGAGGATGGAGCTGA170890.46027021516535005No Hit
AAGGTGCGAACAGATA165280.4451604023788932No Hit
TTTCGTAACAGTACGG162480.43761896284198076No Hit
TGGTCCTTGGAGAGGG159370.42924257821348144No Hit
TCCCAGACTGTCTTGA158920.42803056114504906No Hit
TCTCTCTACTCTGGAT155410.4185768280112766No Hit
GCGGTTCGAGTACGTT154550.4162605287249392No Hit
TCCTTCACTTGTCACA136750.36831852024028106No Hit
TCGCCGAGAAGGTAAT135970.36621769065499826No Hit
TTCGATGGACAGGTGA134480.3622045674728555No Hit
TGATATCCTCGATGTT133860.360534677289682No Hit
AGCTGCTCTTGTCACA132840.35778743860123535No Hit
AACTCCCTGCTTCACC130260.3508385407422231No Hit
CTTTCGGTGCTTTGTT128420.3458827376179663No Hit
AGCCCAGTGAAAGTAC123680.3331161578304787No Hit
TCGGCTTACCTCCTAC120880.32557471829356616No Hit
AACCCTCACGCGGTTC120680.3250360440409295No Hit
ATGGCCGTGATACGCT118730.31978397007772263No Hit
CCCTTGCCTCCTACGG118330.3187066215724494No Hit
TGTTTGTTGCAAGTTC113970.3069635228649713No Hit
AAACACGCTGCAGTTT113550.3058323069344344No Hit
GGGGGGGGGGGGGGGG112240.30230399057966467No Hit
CTAACTGGAACCTTGA108520.2922846494806237No Hit
CAATGTGTGGTTGACT105960.28538961904687515No Hit
TGGCCTTTGCGAAAGC105820.28501254707002954No Hit
TCAAAGGCTCTTAGTG104300.2809186227499913No Hit
TACAACGTGAGCAGGT103800.2795719371183998No Hit
AAATAGGACTCTATCT103460.2786561908889176No Hit
ACAGCCGACAAACATG99340.26755950128460343No Hit
GGACATTTGGCCGCTA99040.2667514899056485No Hit
ACCCTTGTGGGCCATC98130.26430052205615195No Hit
CTTTCGGTGAGCCATT97370.26225355989613286No Hit
TGAACTGTGTGCTGGC96180.259048448092945No Hit
AGTGTTCGATTCGCCT91790.24722454824757145No Hit
GAGGTTGGAGGAATAT90490.24372316560543353No Hit
CAGCCGATGTAATAGG89670.24151460116962342No Hit
GATAAGAACATTGGTC86120.2319531331853236No Hit
GGCTTGCGAATACGCT85560.2304448452779411No Hit
GTGCTAATGGGAGTCA81490.2194828242367861No Hit
GTAAAGCCTGAAATCA79480.21406914799778823No Hit
CGACGTTCTTGCTACA78940.21261472751566934No Hit
TGTACTGGAAATGCTC76420.2058274319324481No Hit
TTGGGAGGAATCTGTG76060.2048578182777022No Hit
GGAACATCTTTACGAC74030.1993902746134406No Hit
AGGGACCCTAATCCTG71310.19206430477758274No Hit
GGGTGAAACCGACTAT71020.19128322711125967No Hit
TCTTGAACTCGTACAT69870.18818585015859918No Hit
TAACGAGCTTACTTTC69790.18797038045754452No Hit
TACGTGTGAAACGCAG68690.18500767206804317No Hit
TACCACCTGAAACATG67830.18269137278170575No Hit
CGGATGAGAACAGGCA67480.1817486928395917No Hit
AGCTTGTGAACCGTGC67070.18064441062168665No Hit
CATGATGTGCCTAGTA65640.1767928897153349No Hit
ACTTATCTGTCAGGTT62770.16906291418999955No Hit
AACTAGCACTGCCACG62440.16817410167314917No Hit
AACTACGACATTGCCA58760.15826249542463558No Hit
ACGTGTTGATCTTTGT58550.15769688745936714No Hit
TCGGACCGAATGTCCA58230.15683500865514857No Hit
GTAGATAGAGCTGAGA58120.15653873781619843No Hit
TGGTAGGGATGTGCTA57810.15570379272461168No Hit
AACGAACCTGACACCC57040.15362989685196074No Hit
CGTCGTACTTGCGCCA57030.1536029631393289No Hit
GGTAGTTTGTCCAGCG55650.1498861107961363No Hit
CTCGGATCTAGTACGG55330.14902423199191772No Hit
TTTAGGGACTGGGCAA54970.14805461833717184No Hit
CCTTCAGTGCAACGCC54770.14751594408453525No Hit
TACCACCTGTACTTTC53370.14374522431607897No Hit
AGTTTGCGAGCAGGAT53220.1433412186266015No Hit
TGAGGAGTGGTCCCAG51950.13992063712235905No Hit
CTTTAACTGTTCGCCT49400.1330525404012423No Hit
CAGGCCATGTCGTAGT48720.13122104794227782No Hit
GAAAGTCCTTATCTGG47900.1290124835064677No Hit
AGAACGCGACACAGTT47750.12860847781699028No Hit
GCCATAATGACCGTTA47730.1285546103917266No Hit
CTTACATTGGAAGGCT47300.1273964607485579No Hit
GAGAAGAGATGCTACA47050.12672311793276214No Hit
GGGATGTGATGCATGT46200.12443375235905656No Hit
CGATCGGTGCGAGGAT45890.12359880726746983No Hit
CAAAGACCTGCTTTGA45370.12219825421061464No Hit
GAAGGACACCCAGCTG44640.12023209318849101No Hit
TGCAATGACTCCGTAA44580.12007049091270001No Hit
TCCTCATCTTTCTGGA43910.1182659321663674No Hit
AATTCTGCTTAGCGAG43810.11799659504004908No Hit
CCGTTACTGTTGGTTG43410.11691924653477587No Hit
GCTGGACACATTGGTC43240.11646137342003475No Hit
AACTACGACGCTGTTA43070.11600350030529363No Hit
GGACCACTGGCTAGAG42250.11379493586948354No Hit
ATGGCCGTGGGCCAAA41310.1112631668820915No Hit
TCCGTAAGATTACGCA41110.11072449262945487No Hit
GGCTTAAACTGCATCA41110.11072449262945487No Hit
TGTCTTCCTGAAATCA40950.11029355322734558No Hit
GCGTTTAGAAGTCTAC40660.1095124755610225No Hit
TCGTAGAACCGCACTG40640.10945860813575885No Hit
CCCACCTCTTGACCTA40380.10875833160733125No Hit
ATAGCCAACATCTGTG40330.10862366304417209No Hit
TATGGGTACCCTTTGC39670.1068460380104713No Hit
ACAAGACCTAAGCTAA39590.10663056830941665No Hit
CCTGAGGACTGACTCA39080.10525694896519329No Hit
AGGTGATCTGTTAGTC38030.1024289091388511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)