FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17401297
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG358390.20595591236676208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG29950.010.935475
CCTACAC22300.08.6612423
TATACCG3601.4733814E-108.5533965
TACGCTG4450.08.4922385
ATACTGT40700.08.4259496
TAGACAG46200.08.3918215
TCTATAC21100.08.3578393
TACACTG38150.08.2914855
CTAGTAC12600.08.2199113
GTGTATA52300.08.1691921
CTATACT25200.08.1087844
GTATAGA25250.08.0997671
CTAAGAC23200.08.0839333
CTACACT27750.08.0697564
GTCTTAC20750.08.0335931
CTTAGAC19750.08.00787453
CTGTGCG9300.07.97960339
TAGGACT23800.07.9388984
GTCCTAA18150.07.8723521
GTATTAG24050.07.8632061
Produced by FastQC (version 0.11.5)