FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674884
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC358040897.42914334166738Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA221240.60203260837621No Hit
ATCTGCTC127470.3468680916186742No Hit
ATCAGATC104220.28360078848747333Illumina PCR Primer Index 9 (100% over 8bp)
ATATGATC73640.20038727753039282No Hit
ATCTGAAC70280.1912441317875612No Hit
AACTGATC62490.1700461837706986No Hit
ATCTCATC54260.14765091904941763No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)