FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15251479
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG27050.09.26096255
TATACTG16300.08.8434145
TAGACTG20650.08.7425885
ATACCGT4400.08.5904716
TAGGACA17300.08.4926864
GTGTAGG24000.08.3403371
CTAATAC17550.08.2914873
GTATTAG14150.08.1117811
TGGACTG24450.08.0706885
CCTACAC22300.07.96847253
TAGACAG25600.07.9268095
CCAGGAC48050.07.8622453
CTATACT16450.07.82571134
CTACACT25050.07.82027774
GTACTAG9900.07.7765381
TAGAACC16850.07.722984
GTCCTAA16400.07.6817031
CTAGGAC23200.07.659353
CCTATAC11900.07.5250473
TCTATAC11800.07.47024353
Produced by FastQC (version 0.11.5)