FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478082
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT446231.8007071598114994No Hit
GAAGCAGTGGAACCTT151780.6124898207565367No Hit
GTTCTTCCTAACGTAA127970.5164074473726051No Hit
CGCTTAAGATAATAGG105580.42605531213252834No Hit
CATGGAGGATCGACAT103060.41588615711667326No Hit
TGTCCCATGAGACAAT100080.4038607277725273No Hit
AAAGCATGATCTTTGT94970.38323994121259913No Hit
GATACGTACCGCTCGA94460.3811818979355808No Hit
TGAGCGCACAGGACAC90080.36350693802707096No Hit
TAATTGCCTGTCTGGC85690.3457916243288156No Hit
TGTCGAAACTTCGCAA84780.34211942946197904No Hit
GGGGGGGGGGGGGGGG71730.2894577338441585No Hit
GGAAGGACTAGGTAAT70300.28368714191055827No Hit
AATGATGGAAAGCTAA69000.27844114924364893No Hit
CATGTCAGAGGTTTGT68140.2749707233255397No Hit
TCGACCTTGTTTCGCC60910.24579493333957472No Hit
GTGAGATCTCGATTAC54080.21823329494342802No Hit
ATGGGTACTTGGCTGC52230.21076784384051858No Hit
AAACGACCTAGGGAGG50060.20201107146575456No Hit
AGCCTACCTTAACTCG48890.19728967806553616No Hit
TTGCGATGACATTCAG46680.1883714905317903No Hit
TGTTCCTCTTTATGGC46180.1863538010445175No Hit
GCTATGATGACCTGCG45200.18239912964946278No Hit
CAGCTCACTGTTTACG45060.1818341765930264No Hit
CAATGTGACCAACGCC43550.17574075434146247No Hit
CTGCCTTGACCATAAC43230.17444943306960786No Hit
CCTGTTGACCCTAGTA43150.1741266027516442No Hit
TAACCAGACATCACAC41920.1691630866129531No Hit
CACAGCTCTGGACTGA41080.16577336827433475No Hit
CATGATGTGTCATGTG41000.1654505379563711No Hit
ATGGGTACTTTCGGGC40710.16428027805375287No Hit
CATGCAAACGAAGGCT40250.16242400372546187No Hit
ATGCCGCGATGCCACG39940.16117303624335272No Hit
TGGGCCATGCGTTCTC39170.1580657944329526No Hit
TTGTTTGTGATACGCT38750.1563709352636434No Hit
CATTAGTCTATTCTGG37730.15225484870960687No Hit
ATTAGCGTGGTCGGTC37710.15217414113011593No Hit
CTATTCAACCGAACGA37670.15201272597113413No Hit
AGAACGCGAGGAATAT37500.15132671154546137No Hit
CCGCTATTGTTCGCAA37040.14947043721717038No Hit
CACTATACTACGCCAC35800.14446656728873378No Hit
AGCTTGTGACTCACTC35510.14329630738611554No Hit
GGGCTGACTAATACAG34270.13829243745767897No Hit
ATAGGAAACAGACTTC34140.13776783819098803No Hit
CCCAATGCTGGAATGC33750.13619404039091523No Hit
TTGACGTGACCTCAAA33420.13486236532931517No Hit
GGTAGCCTGTGCCACG33110.13361139784720602No Hit
GCAGATACTCGGCTCA32870.13264290689331507No Hit
AACCAACACCGGAATG32760.13219901520611504No Hit
AGCTCGGACCTTAAGC32700.13195689246764233No Hit
CTGATTACTGCTTTGA32660.13179547730866048No Hit
AACGTCCCTTACAGAA32580.13147264699069683No Hit
CCTACGGGATGGAACG32370.13062521740604224No Hit
CTGCCATTGCACAGTT32280.13026203329833314No Hit
TTGTAACCTCATCCTC31960.12897071202647856No Hit
CGCTTACTGGCCACGT31920.1288092968674967No Hit
TGTTTGTTGCGCTCGA31870.12860752791876942No Hit
ATAGCCAACATGGCCC31670.12780045212386032No Hit
CGATCAAACCGATCGC31530.1272354990674239No Hit
GCATCTCTGAGCCTAT31530.1272354990674239No Hit
GGGATCAGATGAGTAA31520.12719514527767847No Hit
TGGAGTTACAAGTCTC31240.12606523916480566No Hit
ATCTTACTGCGCACTG31140.1256617012673511No Hit
CGCTGAGTGTTAGCTG30870.1245721489442238No Hit
TCCAAAGTGAAGGCCA30160.1217070298722964No Hit
TCAGGCTCTTAAAGCA30030.12118243060560546No Hit
CGCTTCCCTGCGCTGT29920.12073853891840544No Hit
AGCCGGTACGCGCAAA29340.11839801911316897No Hit
TTCTAACACTACGTTG29150.11763129710800531No Hit
TGGGTGCACTTCTGAG29060.1172681130002962No Hit
CTCGGATCTTTCTGAG29040.11718740542080527No Hit
GATACCATGTCTGGAT28970.11690492889258709No Hit
ACCTGCTTGCCAAACT28760.11605749930793251No Hit
CATGTTACTAACGTGG28250.11399945603091423No Hit
TACGCCTTGGGTTGAG28130.11351521055396877No Hit
CGAGTCTCTTACTTTC27970.11286954991804145No Hit
CTTAGGCCTTATGACC27930.11270813475905964No Hit
ATTGAGAGATGATCAC27760.11202212033338686No Hit
AGTGGTACTCAACGCC27550.11117469074873229No Hit
ATCGCAATGTAACTCG27540.11113433695898683No Hit
CCTTCAGTGTAGCTTG27510.11101327558975045No Hit
TACAACCGATTCCATT27340.1103272611640777No Hit
TGGTCCTTGTTTCTGT27290.11012549221535042No Hit
CATGTCAGAACAGGTG27280.11008513842560497No Hit
AGAACAATGAGTCGGA27270.1100447846358595No Hit
AACAGCTACTCGAGAG27200.10976230810764132No Hit
CCTCCCTTGTTTCGCC27130.10947983157942312No Hit
GTCCTGACTTTACGAC27050.10915700126145947No Hit
AACCACTGAAAACATG26540.10709895798444119No Hit
TTCTATGACCAACGCC26290.10609011324080478No Hit
TGAGATAGACTCATCC26230.10584799050233204No Hit
GCATGGGCTATGTGGG26050.10512162228691382No Hit
GTTGCTAGACGCTCGA26050.10512162228691382No Hit
TCCGGGATGCGAGTTT26020.10500056091767747No Hit
AGGTTACTGGTCCCGA25980.10483914575869564No Hit
GTGTACTGAATCACAC25890.10447596165098652No Hit
TATCTCCCTGGAATTA25800.10411277754327741No Hit
TTCACGCTGTGGGCAA25750.10391100859455013No Hit
AGAACATGAAAGCTAA25740.10387065480480467No Hit
GCGTCGATGCTTGAAC25400.10249862595345915No Hit
AGAGAATTGCCCGCAA25180.10161084257905913No Hit
AAAGGATACTGCTGGC25130.10140907363033184No Hit
CCTTCTTTGGATCATC25070.1011669508918591No Hit
ACCTCCATGGAATTGA25070.1011669508918591No Hit
TCGACCTTGGGACCTC25050.10108624331236818No Hit
TCCCAGACTCTCATTT24800.1000773985687318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)