FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252697
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC286890.5461765641536148No Hit
CTCCTAGTGAACGTAA273920.5214844869216708No Hit
CGACCAGACGCGCTGT256840.4889678578452174No Hit
TAGCTTTACCCTATCC238520.4540905367280847No Hit
CCAGGACTGTAACTCG187110.3562170062350827No Hit
ATCTGTACTGGGACTA171410.32632759894583674No Hit
CTTCCTTTGGCTCAGC163570.31140193314025155No Hit
GTTCTCGACCGTAGAG160350.3052717489701005No Hit
CGATCAAACCCGGCTA153720.2926496616880814No Hit
AGACCATTGAATCCAC140640.26774816822672237No Hit
TGCTGCTACTTTCTTG134840.25670622158483536No Hit
GGGGGGGGGGGGGGGG129280.24612118307985403No Hit
ACAGCCGACTTGCGTA122520.23325160389034433No Hit
AACTTACCTCACCTAA112920.21497527841411757No Hit
CGCGTTGGAGCGGTTC109520.20850241314128723No Hit
ATCGAAAGATTTCCGC106620.20298143982034372No Hit
CCCTGAACTAGCTTTG102220.19460479064373978No Hit
AGTGGAGTGAGTTGAA99650.18971206601104157No Hit
GTAACTGTGTGAAAGT98590.18769405507304152No Hit
CAATTCGGATAACGGG95570.18194462768364517No Hit
GGCAACAGAATAGCAA90250.1718164973155695No Hit
GGGTAGAACCAGTCTA88110.16774239976149397No Hit
AAGAACCCTTAACCCA87710.1669808861999845No Hit
TTCCTAAACTAATGCC84740.16132664800577684No Hit
CGCGATCGATATCTGG83590.15913729651643718No Hit
TTTAGTCTGAGCAATA82540.15713832341747488No Hit
TCCGGGAACGTTCTCC81750.1556343341334937No Hit
GTGAAGCGATCCCAGG81320.15481570705487105No Hit
TGGAACACTCCTAGTA80580.15340690696607856No Hit
TGCAGATACTGCCACG78160.1487997499189464No Hit
AGTCCTGCTCGAACGA75570.1438689496081727No Hit
CTTTAACTGTCCGTAA74570.1419651657043991No Hit
TGCAGTATGACACCGC74270.141394030533267No Hit
TGATAGAGAGACTGTT74180.14122268998192739No Hit
TCTCAGAGATGCATGT72310.13766261408187072No Hit
CATAGGTACAAGGTGC72040.13714859242785182No Hit
AGGTGTGGATCATGTG71910.13690110052036125No Hit
CACATCCTGAGCCTAT71770.13663457077383295No Hit
CTATTGGGACTTGTGA70840.13486405174332347No Hit
ATTTCTGACGGGTAGT70300.13383600843528573No Hit
TCAAAGCACTCTGCAA70000.13326487326415362No Hit
CTTAGATCTTGTCACA68970.1313039758432668No Hit
GCAACTTGAAGGTGCA67510.1285244513437573No Hit
TGGCAATTGAGTGGTG66910.12738218100149312No Hit
TACGTTTACTGCACCG66900.1273631431624554No Hit
AGAGTACCTTAATCGC66880.1273250674843799No Hit
CGAGTCTCTGCTACGC66810.1271918026111158No Hit
TGAACTGTGAACGTAA66130.12589722955654972No Hit
TGAGGGATGTACGTTG65730.12513571599504025No Hit
ATGTACGTGTACTTTC65730.12513571599504025No Hit
TCTATGGGATGCTCAA65660.1250024511217761No Hit
TTCTTAGACGTCCCGA63400.12069989949924773No Hit
GGTCTAAACTCCTACC63020.11997646161581374No Hit
TACTGTTTGCTTAAGC62210.11843439665375712No Hit
GAGTGTTACACCTGCG62060.11814882906819107No Hit
TCCATTTGAAATCTGC61490.1170636722430401No Hit
GGGTGTGCTCGCAAAG61020.1161688938082665No Hit
CTACAACGAATCTGTG60950.11603562893500236No Hit
GGTCGCATGGTACTAC60300.11479816939754948No Hit
CTCAATTTGGAATCTC60210.11462682884620987No Hit
CACCAGGTGCGCCACA59430.11314187740126644No Hit
CTAGCTCACGAATAGT59430.11314187740126644No Hit
TTGCGGTCTTTTGGGT59070.11245651519590794No Hit
CATTCTATGCCTAGCG58950.11222806112745509No Hit
GGGATGTGACTGGAAG57840.11011486099426637No Hit
TCGGACCGATAGGCGA57830.11009582315522864No Hit
TCCGGGTGAACACCTA57390.10925815823756824No Hit
CACATTACTGATGGAC57270.10902970416911541No Hit
CGAGTCTCTTGGAACG56780.10809685005626632No Hit
CTTTCGTCTCGAACGA56710.10796358518300218No Hit
ACCAAGTTGAAGCTAA56700.10794454734396446No Hit
AGCCAATTGTTATGGC56420.10741148785090783No Hit
TGTAAGCACGCGCTGT56160.10691650403592669No Hit
AGACTACTGGCCCTAG55950.10651670941613423No Hit
GTAATCTCTACACAAA55710.10605980127922855No Hit
ACCTCAGCTAATCCGT55600.10585038504981345No Hit
CATAGTCGAAGATTCC54780.10428928224871908No Hit
TGCGCAGACATCAGGG54700.10413697953641719No Hit
TGCTAGTGAAGGCGTA53930.1026710659305115No Hit
ATGTCCAGACCGCTAG53800.10242357402302095No Hit
CTAACAGTGCATGATC53490.10183340101285111No Hit
TAGTACCGAACGAGCA53430.1017191739786247No Hit
AGGCGATGACCTGCGA53100.1010909252903794No Hit
ATAGCCAACCGTTCTC52580.1001009576604171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)