FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959769
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT284674696.1813573964725No Hit
CTTTCCTA540721.8268993289679025No Hit
CTTTCCAT79270.26782495525833266No Hit
CTTTACTT62510.21119891450988235No Hit
CTTTCATT58230.19673832653832107No Hit
CATTCCTT48570.16410064434082525TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT43910.1483561723904805No Hit
ATTTCCTT40260.13602412891006022No Hit
CTTACCTT33370.11274528518948608No Hit
CGTTCCTT33150.11200198393861144No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)