FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544931
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG243514595.6860912928484No Hit
GGCCAGGG347481.3653808295784835ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA285031.1199910724495084No Hit
GGACAGGC53150.20884652668382758No Hit
GGACACGG47440.1864097690664305No Hit
CGACAGGG36000.14145766623928113No Hit
GGAAAGGG35850.14086825929661748No Hit
GGACAGCG34600.1359565347744202No Hit
GCACAGGG31800.12495427184469834No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)