FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15896063
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG17100.010.233535
TACACCG7400.09.0807375
TATACCG2801.0613803E-88.9996595
TAGACTG21350.08.7209815
TGCACCG18200.08.6919785
GTCTAGA16000.08.4930851
TACACTG27300.08.3073785
GTCGTAC2901.763583E-78.2122971
CTATACG3105.995025E-88.1288014
TCTATAC11500.07.913593
CTATACT17250.07.87223964
CTACACT26800.07.8355984
TAGACAG25550.07.8353195
CTTATAC12700.07.82731253
TCTAGAC16150.07.80239773
CTAGGAC24800.07.7344063
GTATAGA14500.07.72922131
GTATAGG13900.07.6597141
TACACAG38000.07.5891865
GTGTAGG25700.07.57696771
Produced by FastQC (version 0.11.5)