FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498721
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG237997295.24760867659894No Hit
GGCCAGGG378621.5152552045626542ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA287861.1520293782299023No Hit
GGACAGGC57170.22879705257209587No Hit
GGACACGG50390.2016631708782213No Hit
GGAAAGGG41790.1672455628299438No Hit
CGACAGGG38780.15519940001304666No Hit
GGACAGCG37720.1509572297187241No Hit
GCACAGGG33180.13278793430719157No Hit
GGTCAGGG32030.1281855797425963No Hit
GGGCAGGG25060.10029130903370163No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)