FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691560
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA457188797.44918534559933No Hit
GCCAAAAA152150.32430577462507143No Hit
CCCAACAA133280.28408461151514636RNA PCR Primer, Index 36 (100% over 8bp)
GCCCACAA112130.239003657632003No Hit
GCCACCAA95000.20249128221742876No Hit
GCAAACAA86860.18514097656216696No Hit
GACAACAA84730.18060090886613409No Hit
GCCAACCA77370.16491316321223645No Hit
GCCAACAC65180.1389303344729685No Hit
TCCAACAA51570.10992079393634527No Hit
ACCAACAA47010.10020121238990867No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)