FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043689
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT334871.1002109611067359No Hit
TGCTGCTACCGTTCTC314341.0327599173240105No Hit
AAACGACCTCTTCAAG301890.9918556067981978No Hit
ATGGGTTACAATCGAA295580.9711241851582076No Hit
TATGCTTTGGACTGTT292570.9612348699226497No Hit
AAAGCGGACTCGGGCT271060.8905640490864869No Hit
GCCTCTTGATCTGTGA266990.8771921178543538No Hit
TCCAAAGTGCTCACGG258610.8496597385606742No Hit
TAGTGCATGAACCCGC242900.7980447411019982No Hit
TGTGGCTCTTACGATC229720.7547420252200536No Hit
TAACTCACTGGTGTCC224390.737230380633501No Hit
GCAGGTCTGGCTGTTA221510.727768178680542No Hit
GAAGTATGAACAGGTG214800.705722562324863No Hit
TTCCCATCTCTTTGTT207420.6814756698204054No Hit
TCACCTGACTCAATGC195280.6415898601992517No Hit
TGAGAATGAGCAGTTT195280.6415898601992517No Hit
GAGCCTGACCGAGTTT189780.6235196828585312No Hit
GGAACTACTACCAAGG184550.6063365869509006No Hit
CTAGAGACTAATCTGC179370.5893177653827313No Hit
TCGTATACTGCTAATC176780.5808083545986465No Hit
ATGTCCAGAGTCGGTC176640.5803483864481555No Hit
TTCGTCCGACCTGTGG176260.5790999014682512No Hit
ATTCCTAACTGCTACA176060.5784428041104068No Hit
TCACACAGATTGGGAG172790.5676992623096513No Hit
AGGTCGAGAAGGGAGG169300.5562329134152668No Hit
TCCGCGAGAGAACAGC165650.5442408866346069No Hit
ACTGATTCTCGGCTCA165140.5425652883721037No Hit
TGGACCTCTAGGGAGG162360.5334316350980669No Hit
TGGCCTGGACCCGAGT161380.5302118580446294No Hit
CCATGATGATTCGGGC160950.528799098725264No Hit
GCAATCATGGAACGTG153300.5036651247877164No Hit
TTCGATGGAAATCCTG142820.46923322323667105No Hit
AAGGTGCGAACAGATA134430.44166798907509935No Hit
TTTCGTAACAGTACGG133820.43966384213367393No Hit
ACGAGGATGGAGCTGA132750.4361483712692065No Hit
TGGTCCTTGGAGAGGG131150.4308915924064515No Hit
TCCCAGACTGTCTTGA131010.43043162425596043No Hit
TCTCTCTACTCTGGAT125750.41314996374465324No Hit
TGCGGTGACCTTAGTG117720.38676750482720146No Hit
TGATATCCTCGATGTT112760.37047149035266086No Hit
AGCTGCTCTTGTCACA112510.3696501186553554No Hit
TTCGATGGACAGGTGA112110.36833592393966663No Hit
AGCCCAGTGAAAGTAC111330.36577324424407354No Hit
TCCTTCACTTGTCACA109650.36025362643818076No Hit
TCGCCGAGAAGGTAAT106460.3497729235805629No Hit
CTTTCGGTGCTTTGTT106190.34888584214747304No Hit
AACTCCCTGCTTCACC105820.3476702120354609No Hit
CCCTTGCCTCCTACGG103030.3385037038935318No Hit
ATGGCCGTGATACGCT100850.3313413426930281No Hit
AACCCTCACGCGGTTC100380.3297971639020938No Hit
TCGGCTTACCTCCTAC97480.32026925221335034No Hit
GGGGGGGGGGGGGGGG96840.3181665406682483No Hit
AAACACGCTGCAGTTT94000.3088357581868581No Hit
GAAGTAATGAGTCTGT93800.30817866082901374No Hit
TGTTTGTTGCAAGTTC91430.3003920571385578No Hit
ACCCTTGTGGGCCATC90210.29638376325570714No Hit
TGGCCTTTGCGAAAGC89030.2925068888444253No Hit
CTAACTGGAACCTTGA87860.2886628693010357No Hit
TACAACGTGAGCAGGT87470.28738152945323914No Hit
CAATGTGTGGTTGACT87260.28669157722750255No Hit
AAATAGGACTCTATCT85200.27992347444170546No Hit
ACAGCCGACAAACATG83030.27279396810909395No Hit
TCAAAGGCTCTTAGTG82720.27177546720443513No Hit
GGACATTTGGCCGCTA81620.26816143173629103No Hit
CTTTCGGTGAGCCATT80410.26418599272133253No Hit
TGAACTGTGTGCTGGC80340.26395600864608704No Hit
CAGCCGATGTAATAGG76380.25094548096076835No Hit
AGTGTTCGATTCGCCT75830.24913846322669628No Hit
GGCTTGCGAATACGCT68530.22515440966537648No Hit
GATAAGAACATTGGTC63700.2092855084734347No Hit
GTAAAGCCTGAAATCA63600.2089569597945125No Hit
GTGCTAATGGGAGTCA62400.20501437564744623No Hit
TTGGGAGGAATCTGTG61660.20258311542342206No Hit
TGTACTGGAAATGCTC61020.20048040387832003No Hit
GGAACATCTTTACGAC59940.19693207814596037No Hit
AGGGACCCTAATCCTG59570.1957164480339483No Hit
CGACGTTCTTGCTACA59040.1939751400356607No Hit
GGACCACTGGCTAGAG58190.19118247626482207No Hit
TACCACCTGAAACATG56660.18615568147731257No Hit
GGGTGAAACCGACTAT56410.1853343097800071No Hit
TAACGAGCTTACTTTC55230.18145743536872525No Hit
TACGTGTGAAACGCAG54930.18047178933195868No Hit
AGCTTGTGAACCGTGC54800.18004467604935984No Hit
TCTTGAACTCGTACAT54450.17889475567313218No Hit
CGGATGAGAACAGGCA54240.1782048034473956No Hit
CATGATGTGCCTAGTA53110.17449220337557483No Hit
ACTTATCTGTCAGGTT52740.17327657326356274No Hit
AACTAGCACTGCCACG50440.16571995364835237No Hit
AACTACGACATTGCCA47660.1565863003743155No Hit
TGGTAGGGATGTGCTA46860.153957910942938No Hit
AACGAACCTGACACCC46220.15185519939783598No Hit
CGTCGTACTTGCGCCA46190.15175663479415932No Hit
ACGTGTTGATCTTTGT45890.15077098875739275No Hit
TCGGACCGAATGTCCA45680.15008103653165614No Hit
CTCGGATCTAGTACGG44680.14679554974243425No Hit
CCTTCAGTGCAACGCC44550.1463684364598354No Hit
AGTTTGCGAGCAGGAT44080.1448242576689011No Hit
GTCCGAGTGGTCTTGA43930.14433143465051784No Hit
GTAGATAGAGCTGAGA43860.14410145057527232No Hit
TACCACCTGTACTTTC43470.14282011072747577No Hit
TTTAGGGACTGGGCAA43200.14193302929438587No Hit
GCCATAATGACCGTTA43000.14127593193654148No Hit
GGTAGTTTGTCCAGCG42350.13914036552354725No Hit
TGAGGAGTGGTCCCAG42090.13828613895834957No Hit
CTTTAACTGTTCGCCT41400.13601915307378645No Hit
AGAACGCGACACAGTT39700.13043382553210922No Hit
CTTACATTGGAAGGCT38750.12731261308234842No Hit
CAAAGACCTGCTTTGA38370.1260641281024441No Hit
CCGTTACTGTTGGTTG37720.12392856168944988No Hit
TGCAATGACTCCGTAA37510.12323860946371328No Hit
CAGGCCATGTCGTAGT36920.12130017225807235No Hit
GGGATGTGATGCATGT36890.1212016076543957No Hit
GAACCATACTTTGGGT36870.12113589791861126No Hit
GAAAGTCCTTATCTGG36160.11880320229826372No Hit
AATTCTGCTTAGCGAG36160.11880320229826372No Hit
TCCTCATCTTTCTGGA35660.11716045890365279No Hit
GTCCGTTACGGATGGT35660.11716045890365279No Hit
CGATCGGTGCGAGGAT35350.11614195799899399No Hit
GAGAAGAGATGCTACA34950.11482776328330523No Hit
AACTACGACGCTGTTA34890.1146306340759519No Hit
CCCACCTCTTGACCTA34700.11400639158599976No Hit
TGTCTTCCTGAAATCA34430.11311931015290985No Hit
GCTGGACACATTGGTC33420.10980096849579574No Hit
GAGGTTGGAGGAATAT33190.1090453065342747No Hit
ATAGCCAACATCTGTG33080.10868390298746028No Hit
ACAAGACCTAAGCTAA33070.10865104811956806No Hit
CCTGAGGACTGACTCA33050.10858533838378362No Hit
ATGGCCGTGGGCCAAA33010.10845391891221474No Hit
TCGTAGAACCGCACTG32960.10828964457275365No Hit
GGCTTAAACTGCATCA31870.1047084639725018No Hit
TCCGTAAGATTACGCA31600.10382138253941188No Hit
TATGGGTACCCTTTGC31150.10234291348426203No Hit
AGGTGATCTGTTAGTC30910.10155439665484878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)