FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583439
Sequences flagged as poor quality0
Sequence length8
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA148530793.8026030683847No Hit
TTAGTCAA709624.4815114443941315No Hit
TCAGGCAA44390.28033918578486444No Hit
TCAGACAA40750.2573512462431455No Hit
TCAGTAAA33160.20941760307785778No Hit
ACAGTCAA33050.2087229125972014TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTCAT19510.12321282979641149No Hit
TAAGTCAA18720.11822368907169772No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)