FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18575064
Sequences flagged as poor quality0
Sequence length34
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCC197940.10656221695925247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA34550.019.44805311
TCCGAGC35500.019.1642613
ACGAGAC34600.019.13952422
ATCTCCG35550.018.86166610
CCCACGA36750.018.24670819
CGAGCCC37650.017.92145215
CACGAGA37000.017.85962721
CCACGAG37300.017.82767120
CTCCGAG39700.017.45415512
CCGAGCC42000.016.53192914
GCCCACG41100.016.24714518
ACTAAGC46850.014.25678327
CTTATAC52400.014.1687151
GACTAAG48000.014.119307526
CTAAGCC48400.013.71297628
GAGACTA50950.013.38356524
TATACAC74900.012.6158533
ACATCTC62650.012.3339488
CGAGACT54300.012.32473823
TACACAT75700.011.5394675
Produced by FastQC (version 0.11.5)