FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004949954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18575064
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1899961.0228551567843858No Hit
TGCTGCTACCGTTCTC1889301.0171162801915514No Hit
AAACGACCTCTTCAAG1871801.0076950475110071No Hit
ATGGGTTACAATCGAA1805320.9719051304480028No Hit
TATGCTTTGGACTGTT1779480.9579940074499878No Hit
AAAGCGGACTCGGGCT1643680.8848852418489648No Hit
GCCTCTTGATCTGTGA1567680.8439701742077443No Hit
TCCAAAGTGCTCACGG1564620.842322804379032No Hit
GCAGGTCTGGCTGTTA1552380.8357333250641829No Hit
TAGTGCATGAACCCGC1493310.8039326270961973No Hit
TAACTCACTGGTGTCC1406980.7574563403926899No Hit
TGTGGCTCTTACGATC1390730.7487080529036132No Hit
GAAGTATGAACAGGTG1344060.7235829712349847No Hit
TTCCCATCTCTTTGTT1268940.6831416570085572No Hit
TCACCTGACTCAATGC1201710.6469479728306723No Hit
TGAGAATGAGCAGTTT1153020.6207354117326325No Hit
GGAACTACTACCAAGG1146870.6174245213906128No Hit
ATGTCCAGAGTCGGTC1100750.5925955356062299No Hit
TCGTATACTGCTAATC1090610.587136604213046No Hit
TTCGTCCGACCTGTGG1088530.5860168234144443No Hit
GAGCCTGACCGAGTTT1082720.5828889741645036No Hit
CTAGAGACTAATCTGC1078440.580584809828919No Hit
TCACACAGATTGGGAG1068800.5753950565123221No Hit
TGCGGTGACCTTAGTG1059250.5702537552495108No Hit
ATTCCTAACTGCTACA1042150.5610478650302363No Hit
GAAGTAATGAGTCTGT1040970.560412604769491No Hit
TCCGCGAGAGAACAGC1040770.5603049335388562No Hit
AGGTCGAGAAGGGAGG1024420.551502810434462No Hit
ACTGATTCTCGGCTCA1003050.5399981394411346No Hit
TGGACCTCTAGGGAGG994110.5351852354317596No Hit
TGGCCTGGACCCGAGT976090.5254840575515648No Hit
CCATGATGATTCGGGC955760.5145392769575384No Hit
TTCGATGGAAATCCTG863090.4646498122429081No Hit
GCAATCATGGAACGTG860180.46308319583717183No Hit
ACGAGGATGGAGCTGA843850.4542918398558411No Hit
AAGGTGCGAACAGATA825670.4445045249911387No Hit
TTTCGTAACAGTACGG818670.44073603191892097No Hit
TCCCAGACTGTCTTGA808370.4351909635412292No Hit
TGGTCCTTGGAGAGGG794630.4277939499966191No Hit
TCTCTCTACTCTGGAT780460.4201654433061442No Hit
TCGCCGAGAAGGTAAT683730.36809025260962763No Hit
TCCTTCACTTGTCACA679970.3660660334736936No Hit
TTCGATGGACAGGTGA678150.36508622527491696No Hit
TGATATCCTCGATGTT673970.3628358965546498No Hit
AGCTGCTCTTGTCACA664980.35799607473761597No Hit
AGCCCAGTGAAAGTAC661800.3562841021705228No Hit
AACTCCCTGCTTCACC650710.3503137324318237No Hit
CTTTCGGTGCTTTGTT644720.3470889790743117No Hit
GCGGTTCGAGTACGTT643700.3465398557980742No Hit
AACCCTCACGCGGTTC638010.34347660928651447No Hit
CCCTTGCCTCCTACGG613320.3301845958646495No Hit
ATGGCCGTGATACGCT611230.32905943150451594No Hit
TCGGCTTACCTCCTAC595140.320397280999947No Hit
TGTTTGTTGCAAGTTC568880.306260048417599No Hit
AAACACGCTGCAGTTT565850.30462882927348195No Hit
GGGGGGGGGGGGGGGG550450.296338144514603No Hit
ACCCTTGTGGGCCATC549600.29588054178440515No Hit
TGGCCTTTGCGAAAGC545730.29379710347162197No Hit
CTAACTGGAACCTTGA535690.28839200769375545No Hit
CAATGTGTGGTTGACT527250.28384828176096727No Hit
TACAACGTGAGCAGGT526140.2832507064309442No Hit
AAATAGGACTCTATCT526020.2831861036925633No Hit
TCAAAGGCTCTTAGTG521800.2809142407261692No Hit
ACAGCCGACAAACATG491780.2647527890078871No Hit
CTTTCGGTGAGCCATT490820.26423596710084013No Hit
TGAACTGTGTGCTGGC483730.2604190219748368No Hit
AGTGTTCGATTCGCCT461290.2483383098976133No Hit
CAGCCGATGTAATAGG453700.24425218669502294No Hit
GGACATTTGGCCGCTA439910.23682825534275415No Hit
GAGGTTGGAGGAATAT407520.21939089954144977No Hit
CGACGTTCTTGCTACA386050.20783239293280498No Hit
TTGGGAGGAATCTGTG385720.20765473540225757No Hit
TGTACTGGAAATGCTC381120.20517829709765736No Hit
GTGCTAATGGGAGTCA377070.20299795467730286No Hit
AGGGACCCTAATCCTG361480.19460498224932093No Hit
GGAACATCTTTACGAC356830.19210162613706203No Hit
TCTTGAACTCGTACAT351790.1893883111250653No Hit
GGACCACTGGCTAGAG346530.1865565577593703No Hit
TACGTGTGAAACGCAG346360.18646503721333071No Hit
TACCACCTGAAACATG344710.1855767495605937No Hit
GGGTGAAACCGACTAT343350.18484458519227712No Hit
TAACGAGCTTACTTTC341720.18396706466260357No Hit
CGGATGAGAACAGGCA341120.18364405097069922No Hit
AGCTTGTGAACCGTGC330970.17817973601598358No Hit
CATGATGTGCCTAGTA320740.17267235256901403No Hit
ACTTATCTGTCAGGTT319620.17206939367745921No Hit
TCGGACCGAATGTCCA298840.16088235281450444No Hit
ACGTGTTGATCTTTGT297910.16038168159205266No Hit
AACTAGCACTGCCACG294430.1585082021790073No Hit
TGGTAGGGATGTGCTA290000.15612328442044668No Hit
AACTACGACATTGCCA284910.15338305160079124No Hit
AACGAACCTGACACCC283740.15275317490157772No Hit
CGTCGTACTTGCGCCA283140.15243016120967337No Hit
CTCGGATCTAGTACGG281530.1515634078030633No Hit
GTAGATAGAGCTGAGA278500.1499321886589462No Hit
TTTAGGGACTGGGCAA277560.14942613387496267No Hit
GTCCGAGTGGTCTTGA277340.14930769552126444No Hit
CCTTCAGTGCAACGCC272980.14696046269342597No Hit
TACCACCTGTACTTTC271870.1463628873634029No Hit
GGTAGTTTGTCCAGCG266040.14322427099039875No Hit
AGTTTGCGAGCAGGAT264680.14249210662208217No Hit
TGAGGAGTGGTCCCAG261810.14094702446247293No Hit
CTTTAACTGTTCGCCT254680.13710854509034262No Hit
GCCATAATGACCGTTA250640.13493358623151985No Hit
GGCTTGCGAATACGCT247520.1332539150336171No Hit
AGAACGCGACACAGTT241950.1302552712604382No Hit
CTTACATTGGAAGGCT240150.12928623018472507No Hit
CAGGCCATGTCGTAGT238650.12847869595496414No Hit
GTCCGTTACGGATGGT235940.12701975077986272No Hit
GGGATGTGATGCATGT233170.12552850423557085No Hit
CAAAGACCTGCTTTGA231510.1246348330213021No Hit
CCGTTACTGTTGGTTG231490.12462406589823863No Hit
CGATCGGTGCGAGGAT230180.12391881933758075No Hit
TGCAATGACTCCGTAA229040.12330509332296245No Hit
TCCTCATCTTTCTGGA220380.118642929036476No Hit
AACTACGACGCTGTTA219540.11819070986780987No Hit
AATTCTGCTTAGCGAG217830.11727012084588241No Hit
GAGAAGAGATGCTACA214850.11566581950942403No Hit
GAACCATACTTTGGGT213760.11507901130246442No Hit
GAAAGTCCTTATCTGG213050.11469677843371091No Hit
CCCACCTCTTGACCTA209090.11256488806714204No Hit
TGTCTTCCTGAAATCA207860.11190270999873808No Hit
TCGTAGAACCGCACTG207010.11144510726854025No Hit
ATGGCCGTGGGCCAAA206250.11103595659212803No Hit
TCCGTAAGATTACGCA204750.1102284223623671No Hit
CCTGAGGACTGACTCA201280.10836032651085348No Hit
GAAGGACACCCAGCTG199820.10757432652721949No Hit
TATGGGTACCCTTTGC198830.10704135393557729No Hit
AGGTGATCTGTTAGTC192570.10367124441670833No Hit
GATAAGAACATTGGTC191820.10326747730182786No Hit
ACAAGACCTAAGCTAA190940.10279372388703478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)