FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18575064
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG379640.20438152999095993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG51100.09.3965125
GCGTAGA3251.7644197E-109.049361
TATACTG33450.08.9140875
TCCGTAC3302.2519089E-98.4835783
TACACTG43200.08.457665
GTGTATA57350.08.4493751
ATACTGT43100.08.44496256
GTCCTAA20450.08.3550391
GTCCTAG23800.08.1793691
GTCTAGC21950.08.1669031
TACACCG4309.094947E-128.1388865
TTACGAC2809.2031587E-77.9988023
TCTATAC22150.07.89949373
ACTGATC28200.07.89471158
TCTAGAC27400.07.81634763
GTGTAAG29400.07.81229161
CTAGACA45000.07.80807734
GTACTGT22950.07.8078046
TCTACAC26500.07.7648743
GTGTAGA27400.07.7180631
Produced by FastQC (version 0.11.5)